Advances in the Biosynthesis of Pyranocoumarins: Isolation and 13 C-Incorporation Analysis by High-Performance Liquid Chromatography-Ultraviolet-Solid-Phase Extraction-Nuclear Magnetic Resonance Data

• Published in: Journal of natural products (Washington, D.C.) Vol. 83; no. 5; pp. 1409 - 1415
• Main Authors: Amaral, Jéssica C, da Silva, Michelli M, da Silva, M Fátima G F, Alves, Thayana C, Ferreira, A Gilberto, Forim, Moacir R, Fernandes, João B, Pina, Edieidia S, Lopes, Adriana A, Pereira, Ana M S, Novelli, Valdenice M
• Format: Journal Article
• Language: English
• Published: United States 22.05.2020
• ISSN: 0163-3864; 1520-6025
• DOI: 10.1021/acs.jnatprod.9b00607

and were obtained by germination from seeds, and isotopic-labeling experiments using d-[1- C]glucose were performed with the seedlings. After 60 days, the seedlings were analyzed by high-performance liquid chromatography-ultraviolet-solid-phase extraction-nuclear magnetic resonance, data and the C enrichment patterns of xanthyletin and seselin indicated that the pyran ring was formed by the methylerythritol phosphate pathway and that the coumarin moiety was derived from the shikimate pathway in both compounds. This information regarding the biosynthetic pathway can be used to increase resistance against phytopathogens, because xanthyletin and seselin are reported to have antimicrobial activity on the growth of , which causes citrus variegated chlorosis in orange.