4B.08: SERUM LEVELS OF TIMP-1 AND IL-6 ARE ASSOCIATED WITH HYPERTENSION AND ATHEROSCLEROSIS IN PATIENTS WITH EARLY STAGES OF CHRONIC KIDNEY DISEASE AND TYPE 2 DIABETIC NEPHROPATHY

• Published in: Journal of hypertension Vol. 33 Suppl 1; p. e55
• Main Authors: Dimas, G, Iliadis, F, Tegos, T, Spiroglou, S, Kanellos, I, Karamouzis, I, Savopoulos, C, Hatzitolios, A, Grekas, D
• Format: Journal Article
• Language: English
• Published: England 01.06.2015
• ISSN: 1473-5598
• DOI: 10.1097/01.hjh.0000467492.91729.d5

Tissue inhibitor of metalloproteinase -1 (TIMP-1) has been identified in humans and its expression is regulated during development and tissue remodeling. TIMP-1 overexpression in a mouse model of atherosclerosis showed a lesion reduction. Interleukine-6 (IL-6) is considered to be pro-inflammatory lipocytokine. The aim of the present study was to determine the serum levels of TIMP-1 and IL-6 and to investigate their potential correlation with hypertension, atherosclerotic markers and albuminuria in early stages of type 2 diabetic nephropathy (DN). CKD patients of stages 1 and 2 with type II DN (n = 50) were included. As controls, there were two groups, patients with diabetes type II without CKD (n = 40) and healthy individuals (n = 40). Clearance of creatinine (Clcr) and albumin excretion were examined in the 24 h urine. TIMP-1 and IL-6 levels were measured by an ELISA method. Blood pressure (BP) was taken using a manual sphygmomanometer. Intima media thickness (IMT) of carotid and femoral arteries and atheromatic plaque were evaluated by a high resolution ultrasonography. Statistical analysis was performed with the use of a SPSS system. There was a statistically significant difference between TIMP-1 (400 ± 20, p < 0.0001), IL-6 (4 ± 0.5, p < 0.0001), BP (20 ± 5, p < 0.0001) and IMT (0.3 ± 0.09, p < 0.0001) between patients and controls. There was a statistically significant negative strong correlation between levels of TIMP-1 and IL-6 (r = -0.7, p < 0.0001), such as between TIMP-1 and IMT (r = -0.65, p < 0.0001) in the patient group. There was also a statistically positive correlation between IL-6 and IMT (r = 0.7, p < 0.0001) in the patient group. Further, TIMP-1 and IL-6 levels were independently correlated with IMT and atheromatic plaque. Our study suggests that serum levels of TIMP-1 and IL-6 might present independent risk factors of blood pressure, atherosclerosis and albuminuria, at least in the early stages of type II diabetic nephropathy to the progression of CKD.