Colistin-Nonsusceptible Pseudomonas aeruginosa Sequence Type 654 with bla NDM-1 Arrives in North America

• Published in: Antimicrobial agents and chemotherapy Vol. 60; no. 3; pp. 1794 - 1800
• Main Authors: Mataseje, L. F., Peirano, G., Church, D. L., Conly, J., Mulvey, M., Pitout, J. D.
• Format: Journal Article
• Language: English
• Published: 01.03.2016
• ISSN: 0066-4804; 1098-6596
• DOI: 10.1128/AAC.02591-15

ABSTRACT This study describes 3 different bla NDM-1 genetic platforms in 3 different species obtained from the same patient who was directly transferred to an institution in Calgary, Alberta, Canada, following a prolonged hospital stay in India. The bla NDM-1 in the Escherichia coli isolate was located on a 176-kb IncA/C plasmid contained within an IS CR1 region. The bla NDM-1 in the Providencia rettgeri isolate was located on a 117-kb IncT plasmid contained within Tn 3000 , while the bla NDM-1 in the Pseudomonas aeruginosa isolate was located on the chromosome within an IS CR3 region. This report highlights the plasticity of the genetic regions and environments associated with bla NDM-1 . To the best of our knowledge, this is the first report of P. aeruginosa with bla NDM-1 identified in North America and the first report of bla OXA-181 in P. rettgeri . The P. aeruginosa isolate belonged to the international high-risk sequence type 654 clone and was nonsusceptible to colistin. This case emphasizes the need for the use of appropriate infection prevention and control measures and vigilant screening for carbapenem-resistant Gram-negative bacteria in patients with a history of travel to areas of endemicity, such as the Indian subcontinent.