Multi-Biomarkers' Responses in Gills of Oreochromis niloticus Exposed to Glyphosate and Polyethylene Microplastic, Isolated and in Mixture

• Published in: Environmental toxicology Vol. 39; no. 11; pp. 5048 - 5058
• Main Authors: Soares, Marco Aurélio Miranda, Ferreira, Ericsson Rubens Rodrigues, Tavares, Driele, Moron, Sandro Estevan, Fernandes, Marisa Narciso, Mariano, Wagner Dos Santos, Paulino, Marcelo Gustavo
• Format: Journal Article
• Language: English
• Published: United States Wiley Subscription Services, Inc 01.11.2024
• Subjects: Anaemia; Anemia; Aquatic environment; Biomarkers; Cell proliferation; Contaminants; Cytogenetics; Damage; Epithelium; Erythrocytes; Exposure; Fish; Gills; Glyphosate; Herbicides; Histopathology; Hypertrophy; Immunosuppression; Marine fishes; Microplastics; Mixtures; Oreochromis niloticus; Parameters; Plastic debris; Plastic pollution; Polyethylene; Polyethylenes; Rivers; Tilapia; Toxicity; Water pollution; osmoregulatory alteration; contaminants interaction; synergism; immunosuppression; freshwater contamination
• ISSN: 1520-4081; 1522-7278; 1522-7278
• DOI: 10.1002/tox.24386

Microplastics (MPs) and glyphosate-based herbicides (GBH) are among the most common contaminants in aquatic environments. In Brazilian rivers, both contaminants were found in elevated levels, leading to a high probability of their association, which can alter their individual effects and potentially intensify their toxicity. This study evaluated the isolated and combined effects of polyethylene microplastics (PE-MPs) and GBH on Oreochromis niloticus using multi-biomarkers of toxicity. The fish were subjected to a 96-h exposure period, with concentrations set based either isolated, PE-MPs group (5 mg L ), GBH group (5 mg L ), or in a group of associated contaminants (GAC), PE-MP + GBH (5 mg L  + 5 mg L ). Toxicity effects were evaluated using biochemical, cytogenetic, hematological, and histopathological biomarkers. We observed change in erythrocyte parameters leading to macrocytic normochromic anemia in GAC. Leukocyte parameters indicate a nonspecific immunosuppression caused by the exposure of associated contaminants, besides the attempts to repair damage caused by PE-MPs. Histopathological markers indicate damage to tissues exposed to contaminants. Besides, there were morphophysiological adjustments on gills, with proliferation and hypertrophy of mitochondria-rich cells on GBH and GAC, besides epithelium ruptures, which were mostly present in the exposed groups. Therefore, this study indicates that PE-MPs and GBHs present toxic effects in O. niloticus with the used concentrations, intensified by the association of contaminants. Thus, multi-biomarkers were useful key to verify toxicity, providing data to the investigation of high levels of contaminant's mixture toxicity present in aquatic environments.