Squalene ameliorates atherosclerotic lesions through the reduction of CD 36 scavenger receptor expression in macrophages
Scope Anti‐atherogenic features of olive oil (OO) have been attributed, in part, to minor compounds, via diverse mechanisms, although its effects on the CD 36 receptor have not been examined. We investigated the effects of minor compounds of OO (squalene ( SQ ), tyrosol ( T yr) and hydroxytyrosol (...
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Published in | Molecular nutrition & food research Vol. 56; no. 5; pp. 733 - 740 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
01.05.2012
|
Subjects | |
Online Access | Get full text |
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Summary: | Scope
Anti‐atherogenic features of olive oil (OO) have been attributed, in part, to minor compounds, via diverse mechanisms, although its effects on the
CD
36 receptor have not been examined. We investigated the effects of minor compounds of OO (squalene (
SQ
), tyrosol (
T
yr) and hydroxytyrosol (
OH
‐
T
yr)), on the expression of the
CD
36 receptor, as well as on monocyte/macrophage differentiation and proliferation.
Methods and results
U
937 monocytic cells and macrophages (obtained with 10 nM phorbol‐myristate‐acetate) were exposed to
T
yr,
OH
‐
T
yr or
SQ
at 0, 10, 75 and 200 μM with/without native or oxidised
LDL
(ox
LDL
). Flow cytometry was used to achieve the expression of
CD
36 in both cell types exposed to ox
LDL
plus antioxidants, as well as the inhibition of monocyte/macrophage differentiation after ox
LDL
and apoptosis.
SQ
caused a dose‐dependent reduction of
CD
36 in the presence of native and moderate
LDL
in monocytes and macrophages. Phenotype‐dependent cytotoxic and antiproliferative effects were found for
OH
‐
T
yr (
p
< 0.05), while
SQ
affected neither monocytes nor macrophages (
p
< 0.01).
Conclusion
SQ
does not prevent monocyte migration and activation into macrophages, but it would inhibit ox
LDL
uptake by macrophages, by reducing
CD
36 expression. This study provides new data about the role of the components of OO in the prevention of atherosclerosis. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 |
ISSN: | 1613-4125 1613-4133 |
DOI: | 10.1002/mnfr.201100703 |