Non-specific Immune Response of Rainbow Trout (Oncorhynchus Mykiss) by Dietary Heat-inactivated Potential Probiotics
Background: The effects of the dietary administration of two heat-inactivated whole bacteria from the Vibrionaceae family, singly or combined, on innate immune response of the rainbow trout were studied. The two bacteria (Pdp11 and 51M6), which were obtained from the skin of rainbow trout, showed in...
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Published in | Immune network Vol. 8; no. 3; pp. 67 - 74 |
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Main Authors | , |
Format | Journal Article |
Language | English |
Published |
대한면역학회
01.09.2008
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Subjects | |
Online Access | Get full text |
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Summary: | Background: The effects of the dietary administration of two heat-inactivated whole bacteria from the Vibrionaceae family, singly or combined, on innate immune response of the rainbow trout were studied. The two bacteria (Pdp11 and 51M6), which were obtained from the skin of rainbow trout, showed in vitro characteristics that suggested they could be considered as potential fish probiotics. Methods: The fish were fed four different diets: control (non-supplemented), or diets supplemented with heat-inactivated bacteria at 108 cfu/g Pdp11, 108 cfu/g 51M6 or with 0.5×108 cfu/g Pdp11 plus 0.5×108 cfu/g 51M6 for 4 weeks. Six fish were sampled at weeks 1, 2, 3 and 4, and then the main humoral (natural haemolytic complement activity and serum peroxidase content) and cellular innate immune responses (leucocyte peroxidase content, phagocytosis, respiratory burst and cytotoxicity) were evaluated. Results: The serum peroxidase contentand the natural haemolytic complement activity increased
with time, reaching the highes t values in the third and fourth weeks of feeding, respectively. The phagocytic ability of specimens fed the mixture of the two inactivated bacteria was significantly higher than in the controls after 2 and 3 weeks of treatment. The same activity increased significantly in rainbow trout fed the Pdp11 diet for 2 weeks or the 51M6 diet for 3 weeks. Respiratory burst activity was unaffected by all the experimental diets at all times assayed. Cytotoxic activity had significantly increased after 3 weeks in fish fed the 51M6 diet. Conclusion: Our results demonstrated the usefulness of incorporating inactivated probiotic
bacteria into fish diets. KCI Citation Count: 0 |
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Bibliography: | G704-001562.2008.8.3.005 http://kmbase.medric.or.kr/Main.aspx?d=KMBASE&m=VIEW&i=0923620080080030067 |
ISSN: | 1598-2629 2092-6685 |
DOI: | 10.4110/in.2008.8.3.67 |