Redox activity ofHydrodictyon reticulatum plasmalemma vesicles

Plasmalemma vesicles with preserved redox activity were prepared from nets ofHydrodictyon reticulatum. Since the walls are mechanically very resistant, a combination of partial cell-wall enzyme digestion and ultrasonic homogenization had to be used for the disruption of cells. To isolate the plasma-...

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Published inFolia microbiologica Vol. 44; no. 4; pp. 419 - 424
Main Authors Matousková, J, Nespůrková, L, Rybová, R, Janácek, K
Format Journal Article
LanguageEnglish
Published United States Springer Nature B.V 01.08.1999
Subjects
Cellular biology
Enzymes
Membranes
Microbiology
Polymers
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Summary:Plasmalemma vesicles with preserved redox activity were prepared from nets ofHydrodictyon reticulatum. Since the walls are mechanically very resistant, a combination of partial cell-wall enzyme digestion and ultrasonic homogenization had to be used for the disruption of cells. To isolate the plasma-membrane-enriched microsomal fraction separation in an aqueous two-phase polymer system was found to be most suitable. The right-side-out vesicles reduced added hexacyanoferrate(III) by electrons supplied either by a transmembrane flow or from external NADH.
Bibliography:ObjectType-Article-1
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ISSN:0015-5632
1874-9356
DOI:10.1007/BF02903716