The Effect of Human Cytomegalovirus on the Formation of CFU-MK In Vitro

• Published in: Journal of Huazhong University of Science and Technology. Medical sciences Vol. 24; no. 2; pp. 134 - 137
• Main Author: 姚军霞 宋善俊 胡丽华
• Format: Journal Article
• Language: English
• Published: China Institute of Heamatology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China 2004
• Subjects: Antigens, CD34 - metabolism; Antigens, Viral - biosynthesis; Antigens, Viral - genetics; Cell Division - drug effects; Cells, Cultured; Cytomegalovirus - genetics; Cytomegalovirus - physiology; Cytomegalovirus Infections; DNA, Viral - biosynthesis; DNA, Viral - genetics; Humans; Immediate-Early Proteins - biosynthesis; Immediate-Early Proteins - genetics; Megakaryocytes - cytology; Megakaryocytes - virology; Molecular Sequence Data; Stem Cells - cytology; Stem Cells - virology; 巨噬细胞; 细胞巨化病毒; 聚合酶链反应; 造血干细胞; cytomegalovirus; megakaryocyte progenitor; polymerase chain reaction
• ISSN: 1672-0733; 1993-1352
• DOI: 10.1007/BF02885411

To investigate the mechanism and the suppressive effect of human cytomegalovirus (HC-MV) on colony forming unit-megakaryocyte (CFU-MK), semi-solid culture system was used to ob-serve the effect of HCMV AD169 strain on CFU-MK's growth of 18 cord blood samples. HCMV DNA and immediate early (IE) protein mRNA in CFU-MK was detected by PCR and reverse tran-scription-polymerase chain reaction (RT-PCR). Our results showed that HCMV AD169 signifi-cantly suppressed the formation of CFU-MK in vitro. Compared with the mock group, the CFU-MK colonies decreased by 21. 6%, 33. 8 % and 46. 3 %, respectively, in all the 3 infected groups (P＜0. 05), suggesting the suppression and the titer of the virus was dose-dependent. Both HCMV DNA and the expression of HCMV IE protein mRNA were positively detected in the colony cells of viral infected group, It is concluded that HCMV AD169 strain could inhibit the differentia-tion and proliferation of CFU-MK by directly infecting their progenitors. There was early transcrip-tion of HCMV IE protein in CFU-MK infected by virus.