Polymorphism of β-lacto globulin gene in Barki sheep breed

• Published in: Biotechnology in animal husbandry Vol. 28; no. 2; pp. 231 - 239
• Main Authors: El-Hanafy, A.A., Sabir, J.S.M., Mutawakil, M.H.Z., Elsoud, Abo, Abdel-Sadek, M.A.
• Format: Journal Article
• Language: English
• Published: 2012
• ISSN: 1450-9156; 2217-7140
• DOI: 10.2298/BAH1202231E

?-LG gene polymorphism at the DNA level has been analyzed by PCR-RFLP and reported two genetic variants of the ? -LG gene in Pag native and Leccese sheep breeds. However, no study has been reported in Barki Egyptian local sheep breed regarding ? -LG polymorphism at DNA level. The association of ?- LG polymorphism with milk production and manufacturing properties of the cheese in sheep have been extensively studied. However, the results are conflicting, indicating either superiority of a given ? - LG variant or absence of relationship. The fingerprinting of ? -LG gene and this relation has not clarified yet in local Barki and other sheep breeds raised in Egypt. Regarding the importance of Barki sheep breed as a local adaptive breed in Egypt, this preliminary study aimed to fingerprint ?-LG gene in the Barki sheep breed, in an attempt to have clear image about genotypes of ?-LG gene in this breed. A total of 60 adult females Barki ewes were fingerprinted for ?-LG gene by the PCR-RFLP method. The sheep are raised by farmers in North West coast (Borg EL-Arab, Alexandria, Egypt) in which sheep are living in natural habitats. The amplified product was observed as 120 bp and the restriction digestion with Rsa I revealed three genotypes, namely AA, and BB at the ?-LG locus. The frequencies of AA, and BB genotypes were 0.45, 0.30, 0.25, respectively. Polimorfizam ? - LG gena na nivou DNK je analiziran koriscenjem PCR-RFLP I dobijene su dve genetske varijante ?-LG gena u kod ovaca autohtone Pag i Leccese rase. Medjutim, nijedno istrazivanje do sada se nije bavilo lokalnom egipatskom rasom ovaca Barki u vezi sa polimorfizmom ?-LG na nivou DNK. Veza izmedju ?-LG polimorfizma i proizvodnje mleka, kao i preradjivackim odlikama sira dobijenog od mleka ovaca je ispitivana u ovoj studiji. Medjutim, rezultati su oprecni, i ukazuju s jedne strane na superirornost date ?-LG varijante ili odsustvo odnosa. Metoda ?fingerprinting? ?-LG gena i odnosa jos uvek nije pojasnjenja kod lokalne rase Barki, kao i drugih rasa ovaca koje se gaje u Egiptu. U vezi sa znacajem Barki rase ovaca kao lokalne adaptivne rase koja se gaji u Egiptu, ova preliminarna studija koja je imala za cilj izdvajanje ?-LG gena kod ovaca rase Barki, u pokusaju da se dobije jasnija slika o genotipovima ?-LG gena kod ove rase. Ukupno 60 ovaca rase Barki je bilo ukljuceno u ispitivanje metodom PCRRFLP. Ovce su odgajane na farmama severno-zapadne obale (Borg EL-Arab, Aleksandrija, Egipat) u prirodnim stanistima. Amplifikovani proizvod je posmatran kao 120 bp a restriktivna digestija sa Rsa I otkrila je tri genotipa, naime AA, AB i BB na ?-LG lokusu. Frekvencije AA, AB i BB genotipova su bile 0.45, 0.30, i 0.25, respektivno. Potrebna su dalja istrazivanja kako bi se dobilo vise informacija o trenutnom stanju in situ ocuvanja zivotinjskih genetskih resursa. To bi otvorilo perspektive u smislu poboljsanja tacnosti selekcije i odgajivackih programa i intenziteta da se obezbedi odn. garantuje loklanim odgajivacima ocuvanje lokalnih rasa..