Determination of genomic regions related citrus chlorotic dwarf disease in clementine mandarin × Minneola tangelo hybrid population by QTL analysis

• Published in: Euphytica Vol. 219; no. 2; p. 24
• Main Authors: Aksoy, Deniz, Yeşiloğlu, Turgut, Serçe, Çiğdem Ulubaş, Ayaz, Furkan
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.02.2023; Springer Nature B.V
• Subjects: Biomedical and Life Sciences; Biotechnology; Citrus fruits; Citrus reticulata; Citrus tangelo; Cultivars; Disease control; Disease resistance; Dwarf disease; Fruits; Geminiviridae; Gene mapping; Genetic markers; Genetic screening; Horticulture; Infections; Life Sciences; Mandarins; Plant Genetics and Genomics; Plant Pathology; Plant Physiology; Plant Sciences; Productivity; Quantitative trait loci; Signs and symptoms; Single-nucleotide polymorphism; Species; Threatened species; Viral infections; Viruses; Citrus; QTL analysis; Mandarin; Linkage maps; Citrus chlorotic dwarf disease
• ISSN: 0014-2336; 1573-5060
• DOI: 10.1007/s10681-023-03154-9

Citrus chlorotic dwarf disease (CCDD) has been associated with loss of productivity in citrus species. Especially in the grapefruit species this loss can reach up to 50%. The causative agent for the disease is Citrus chlorotic dwarf associated virus . Some species in the citrus family can develop resistance to the virus and overcome the symptoms associated with the disease but in those cases they may act as the source of the virus to the surrounding citrus species. Virus management studies mainly focus on the introgression of available virus resistance genes into valuable plant cultivars. In this study, we focused host based aspect of the resistance against the virus. Clementine mandarin × Minneola tangelo hybrid population was created in the Alata Horticultural Research Institute field in 2008. This population showed a resistance to the disease development and we analyzed 186 individuals with a total of 2806 markers. DArT-SNP markers were utilized for the mapping studies. According to our results 9 linkage groups were obtained for Clementine and Minneola. The total length of these groups was 798.70 cm for Clementine and 423.7 cm for Minneola. The total number of markers in the linkage groups was 1536 for Clementine and 1270 for Minneola. The average distance between two markers was 0.51 cm and 0.33 cm for Clementine and Minneola, respectively. 15 QTL loci (with LOD ≥ 3) were associated with CCDD resistance. All of these QTLs’ were specifically determined in Clementine linkage map on LG2, LG3, LG5, LG6 and LG8. Host based genetic characteristics that enable the resistance to the disease were identified by QTL based analysis. These results can pave the way of genetic screening of the resistant versus vulnerable species as well as individuals within the species. Moreover, based on this data genetic manipulations or hybridizations based on the genetic markers can be considered in the future to increase the productivity and to decrease the losses associated with CCDD.