Single Oral Dose Toxicity Test of Persicae Semen Aqueous Extracts in Mice

• Published in: The Korea Journal of Herbology Vol. 28; no. 3; pp. 17 - 24
• Main Authors: Cho, Hun-Bum, Park, Ji-Ha, Seo, Bu-Il, Cho, Su-Yeon, Park, Kyu-Ryul, Choi, Seung-Hoon, Han, Chang-Kyun, Song, Chang-Hyun, Park, Soo-Jin, Ku, Sae-Kwang
• Format: Journal Article
• Language: English
• Published: 대한본초학회 30.05.2013
• Subjects: 한의학
• ISSN: 1229-1765; 2288-7199
• DOI: 10.6116/kjh.2013.28.3.17

Objectives : This study was to evaluate the single dose toxicity of Persicae Semen (PS) in ICR mice. Methods : Aqueous extracts of PS (Yield = 18.60%) were administered as an oral dose of 2,000, 1,000 and 500 mg/kg (body weight) according to the recommendation of Korea Food and Drug Administration (KFDA) guidelines (2009-116, 2009). Animals were monitored for the mortality and changes in body weight, clinical signs and gross observation during 14 days after dosing, upon necropsy; organ weight and histopathology of 12 principle organs were examined. Results : Amygdalin contents in PS aqueous extracts were detected as 32.50±5.96 µg/ml. We could not find any PS extracts treatment related mortalities, clinical signs, changes on the body and organ weights, gross and histopathological observations up to 2,000 mg/kg in both female and male mice, except for transient and slight loss of locomotion detected in female and male mice treated with 2,000 mg/kg. In addition, pharmacological immunomodulatory effects related findings were also demonstrated in 2,000mg/kg treated female and male mice as hypertrophy and hyperplasia of lymphoid cells in the submandibular lymph nodes. Conclusions : Based on the results of this experiment, the approximate lethal dose (ALD) of PS extracts after single oral treatment in female and male mice were considered above 2,000 mg/kg, respectively. It should be carefully used in clinics because the possibilities of respiratory or neurological disorders were observed when administered over 2,000 mg/kg of PS extract related to amygdalin. KCI Citation Count: 0