Oxidative Damages of Two Neonicotinoid Pesticides to Arthrospira platensis (Gomont)

• Published in: Journal of limnology and freshwater fisheries research Vol. 9; no. 1; pp. 17 - 28
• Main Authors: TUNCA, Hatice, KÖÇKAR, Feray, DOĞRU, Ali, GÜZEL, Uğur, DİNÇ, Tarık, ONGUN SEVİNDİK, Tuğba
• Format: Journal Article
• Language: English
• Published: Egirdir-Isparta Egirdir Fisheries Research Institute 28.04.2023
• Subjects: Arthrospira platensis; Chlorophyll; Chlorophyll a; Growth rate; Hydrogen peroxide; Imidacloprid; Insecticides; Oxidative stress; Pesticides; Proline; Thiacloprid
• ISSN: 2149-4428; 2148-9300; 2149-4428
• DOI: 10.17216/limnofish.1178160

In this study, chlorophyll-a amount, OD 560 and antioxidant parameters (total SOD, APX, GR, MDA, H2O2 and Proline) were determined in order to understand the effects of Thiacloprid and Imidacloprid on Arthrospira platensis Gomont. Both Imidacloprid and Thiacloprid applications showed significant reductions in growth rate and chlorophyll-a content of A. platensis cultures with dose-dependent manner when the days and concentrations were compared each other. SOD activity significantly decreased in the Imidacloprid application while Thiacloprid caused a significant increase only at 75 µg mL-1 concentration. APX activity significantly increased in the Imidacloprid and Thiacloprid applications at 50 µg mL-1 and 35 µg mL-1 concentrations, respectively. Imidacloprid treatment increased GR activity at 20 and 30 µg mL-1 concentrations while GR activity increased at 15, 25 and 35 µg mL-1 Thiacloprid concentrations. The MDA content of A. platensis cultures did not change with Imidacloprid or Thiacloprid applications. The H2O2 content did not change at all different Imidacloprid concentrations. However, the H2O2 content decreased at 15 µg mL-1 and increased at 45 and 75 µg mL-1 Thiacloprid concentrations. Free proline content increased in the Imidacloprid and Thiacloprid applications at 100 µg mL-1 and 75 µg mL-1 concentrations, respectively. These neonicotinoid pesticides cause oxidative stress in A. platensis cells.