Lycium barbarum polysaccharides protected human retinal pigment epithelial cells against oxidative stressinduced apoptosis

• Published in: 国际眼科杂志：英文版 no. 1; pp. 11 - 16
• Main Author: Lian Liu Wei Lao Qing-Shan Ji Zhi-Hao Yang Guo-Cheng Yu Jing-Xiang Zhong
• Format: Journal Article
• Language: English
• Published: 18.02.2015
• Subjects: age-related; apoptosis; barbarum; cell; degeneration; epithelial; lycium; macular; pigment; polysaccharides; retinal
• ISSN: 2222-3959; 2227-4898
• DOI: 10.3980/j.issn.2222-3959.2015.01.02

AIM: To investigate the protective effect and its mechanism of lycium barbarum polysaccharides(LBP)against oxidative stress-induced apoptosis in human retinal pigment epithelial cells.METHODS: ARPE-19 cells, a human retinal pigment epithelial cell lines, were exposed to different concentrations of H2O2 for 24h, then cell viability was measured by Cell Counting Kit-8(CCK-8) assay to get the properly concentration of H2O2 which can induce half apoptosis of APRE-19. With different concentrations of LBP pretreatment, the ARPE-19 cells were then exposed to appropriate concentration of H2O2, cell apoptosis was detected by flow cytometric analysis. Expression levels of Bcl-2 and Bax were measured by real time quantitative polymerase chain reaction(RT-PCR) technique.RSULTS: LBP significantly reduced the H2O2-induced ARPE-19 cells’ apoptosis. LBP inhibited the H2O2-induced down-regulation of Bcl-2 and up-regulation of Bax.CONCLUSION: LBP could protect ARPE-19 cells from H2O2-induced apoptosis. The Bcl-2 family had relationship with the protective effects of LBP.