Three AFLP markers tightly linked to the genic male sterility ms ₃ gene in chili pepper (Capsicum annuum L.) and conversion to a CAPS marker

• Published in: Euphytica Vol. 173; no. 1; pp. 55 - 61
• Main Authors: Lee, Jundae, Yoon, Jae Bok, Han, Jung-Heon, Lee, Won Phil, Kim, Sang Hoon, Park, Hyo Guen
• Format: Journal Article
• Language: English
• Published: Dordrecht : Springer Netherlands 01.05.2010
• Subjects: amplified fragment length polymorphism; androecium; Capsicum annuum; cleavage amplified polymorphic sequences; gene segregation; genetic markers; genetic polymorphism; genic male sterility; hot peppers; linkage (genetics); loci; plant fertility; vegetable crops
• ISSN: 0014-2336; 1573-5060
• DOI: 10.1007/s10681-009-0107-1

Genic male sterility (GMS) has long been used as a tool for hybrid seed production in chili pepper (Capsicum annuum L.). We developed DNA markers linked to the GMS ms ₃ gene in a segregating population using bulked segregant analysis (BSA) and amplified fragment length polymorphism (AFLP) techniques. The segregating population was subjected to BSA-AFLP with 512 primer combinations. Three AFLP markers (Eagg/Mccc₂₇₆, Eagc/Mctt₁₇₈, and Ecag/Mtgc₂₀₄) were identified as tightly linked to the ms ₃ locus. Among them, we converted the AFLP marker Ecag/Mtgc₂₀₄ to the cleavage amplified polymorphic sequence (CAPS) marker, named GMS3-CAPS, based on sequencing analysis of internal and flanking regions for the markers between male-fertile and sterile plants. This marker will be useful for pepper breeding using the GMS system.