Screening and identification of the tumor‐associated antigen CK 10, a novel potential liver cancer marker

• Published in: FEBS open bio Vol. 7; no. 5; pp. 627 - 635
• Main Authors: Zhu, Zhaoyu, Liu, Zheng, Liu, Yuchun, Wang, Chunyan, Li, Ruyu, Liu, Hui, Gu, Baohua, Li, Guodong, Zhang, Shoutao
• Format: Journal Article
• Language: English
• Published: Amsterdam John Wiley & Sons, Inc 01.05.2017
• Subjects: Antigen (tumor-associated); Antigens; Cell culture; Disease; Etiology; Experiments; Fatalities; Gel electrophoresis; Hepatitis; Hepatocellular carcinoma; Hepatocytes; High-performance liquid chromatography; Identification; Immunoglobulins; Immunotherapy; Invasiveness; Liver cancer; Lysates; Malignancy; Monoclonal antibodies; Proteins; Sodium lauryl sulfate; Tumors; China
• ISSN: 2211-5463; 2211-5463
• DOI: 10.1002/2211-5463.12122

Hepatocellular carcinoma ( HCC ) is a malignancy that is associated with high mortality rates in Asia. These tumors are highly invasive and their etiology is frequently unknown. Thus, most patients are diagnosed in the middle and late stages of the disease, and thus do not have sufficient time for therapy. Therefore, it is essential to study the early diagnosis and treatment of HCC ; in this regard, the study of tumor‐associated antigens has received much attention. Here, antigens from the human primary HCC cell line, QGY ‐7703, were used to immunize mice in order to prepare monoclonal antibodies. The specific antigen recognized by antibody 11C3 was purified from total protein lysates of QGY ‐7703 by immunoaffinity chromatography. The validity of the candidate antigen as a new HCC ‐associated marker was tested using SDS / PAGE , western blot, HPLC ‐ ESI ‐ MS / MS , and RT ‐ qPCR . Our results showed that the levels of CK 10 in HCC ‐derived cell lines were significantly higher than those in normal liver cells. Thus, we suggest that CK 10 may be involved in the formation and development of HCC , and may be a therapeutically targetable tumor‐associated antigen.