Biological relationship of Bean common mosaic virus (BCMV) infecting cowpea with leguminous plant species

Bean common mosaic virus (BCMV) associated with cowpea mechanically inoculated to different legumi-nous plants. Out of nineteen including cowpea Var.C-152, the virus was easily transferred to ten different legumi-nous hosts. All other hosts assessed for the presence of BCMV were found to be uninfect...

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Bibliographic Details
Published inJournal of applied and natural science Vol. 9; no. 4; pp. 2170 - 2174
Main Authors Manjunatha, N., Rangaswamy, K. T., Nagaraju, N., Reddy, M. Krishna, Prameela, H. A., Manjunath, S. H.
Format Journal Article
LanguageEnglish
Published Haridwar Applied and Natural Science Foundation 01.12.2017
Subjects
Beans
Coat protein
Cowpeas
Distortion
Flowers & plants
French beans
Inoculation
Leaves
Leguminous plants
Phaseolus vulgaris
Plant species
Plant virus diseases
Polymerase chain reaction
Primers (coatings)
RNA-directed DNA polymerase
Species
Vegetables
Vigna umbellata
Vigna unguiculata
Viruses
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Summary:Bean common mosaic virus (BCMV) associated with cowpea mechanically inoculated to different legumi-nous plants. Out of nineteen including cowpea Var.C-152, the virus was easily transferred to ten different legumi-nous hosts. All other hosts assessed for the presence of BCMV were found to be uninfected. The number of days taken for symptom expression and symptoms were varied within plant species. Pole bean expressed mosaic symp-tom after long incubation period (15-18 days) whereas, shorter incubation period was observed in common bean and rice bean (7- 10 days). BCMV produced chlorosis, mosaic, leaf distortion, puckering, vein banding, vein clearing and vein netting on cowpea(C-152). A typical virus symptom, mosaic was observed in green gram, common bean, lime bean, rice bean and yard long bean, whereas, leaf rolling and leaf distortion was observed in black gram, pole bean and snap bean. The virus-host relationship was confirmed by back inoculation test to C. amaranticolor. Further symptomatic plants were subjected for Reverse Transcriptase polymerase chain reaction (RT-PCR) for molecular confirmation using BCMV coat protein (CP) specific primer pair. A PCR fragment size of 439bp was amplified for the symptomatic plants. The results generated indicated the ability of a plant to support virus expression and host speci-ficity of BMCV within the leguminous plant species.
ISSN:0974-9411
2231-5209
DOI:10.31018/jans.v9i4.1505