Expression of hENT1 and ERCC1 genes in tumor tissues non-small cell lung cancer

• Published in: 亚太热带医药杂志：英文版 no. 11; pp. 908 - 911
• Main Author: Jia-Jia Wu Shun-Chang Jiao
• Format: Journal Article
• Language: English
• Published: 01.11.2013
• Subjects: cancer; cell; Chemotherapy; ERCC1; Gemcitabine; hENT1; lung; Non-small
• ISSN: 1995-7645; 2352-4146
• DOI: 10.1016/S1995-7645(13)60162-5

Objective:To investigate the expression of hENT1 and ERCCl genes in tumor tissues non-small cell lung cancer(NSCLC).Methods:Fresh non-small lung cancer specimens were transplanted into nude mice.Twenty mice were randomized into two groups:experimental group receiving gemeitabine plus cisplatin and control group receiving 0.9%physiological saline.The expressions of hENTi and ERCC1 mRNA in tumor tissue were detccted by real-time fluorescent quantitative PCR.The volume of tumor,the weight of nude mice and tumor volume were respectively measured and calculated 2-3 times per week.Tissue samples were collected from NSCLC mice treated with gemeitabine plus carboplatin.Results:The histological examination showed that many tumor cells were well preserved in nude mice.The rate of transplanted tumor cells was 86.7%.The concomitant treatment study showed that the rate of TV,RTV,T/C in GEM + DDP group was the lowest.LBP + DOC,DDP + DOC obviously influenced the body weight.Compared with NS group,DDP group,GEM group,the survival period and the level of hENTl of DDP+GEM group increased obviously,the level of ERCC1 decreased significantly(P<0.05).Conclusions:The expression of hENT1 and ERCC1 genes in tumor tissues were closely correlated with the response to chemotherapy and prognosis of patients with NSCLC treated with gemeitabine plus cisplatin.