Rapid and Non-Invasive Bio-Photonic Techniques for Morphological and Functional State Study of Lycopene in Tomato and Tomato Products

• Published in: Russian agricultural sciences Vol. 46; no. 5; pp. 459 - 467
• Main Authors: Hafeez Ullah, Andleeb, Farah, Malik, Sadia, Asim, Muhammad, Nazir, Alia, Batool, Zahida
• Format: Journal Article
• Language: English
• Published: Moscow Pleiades Publishing 2020; Springer Nature B.V
• Subjects: Agriculture; Biomedical and Life Sciences; Crop Production; Life Sciences; Lycopene; Morphology; Optical Coherence Tomography; Plant Biochemistry; Plant tissues; Qualitative analysis; Spectrophotometry; Tomatoes; lycopene concentration; plant photonics; tomato and its products; optical coherence tomography; food quality; Lycopersicon esculentum; spectrophotometer; 2D imaging
• ISSN: 1068-3674; 1934-8037
• DOI: 10.3103/S1068367420050092

The natural red pigment compound of tomato is lycopene which is responsible for red pigment in some fruits and vegetables. We have determined the lycopene concentration from the measured absorbance in fresh tomatoes with and without peel with different concentrations using spectrophotometric method. The measured lycopene contents vary from 3 to 33.5 mg/kg for spectral range of 444 to 560 nm. For qualitative analyses, optical coherence tomography (OCT) was utilized for vital visualization of plant tissues in tomato ( Lycopersicon esculentum Mill). The obtained OCT slices permitted the morphological and practical condition of plant tissues to be surveyed in vitro . The OCT technique permitted plant morphological structures to be related to more prominent confidence. The morphological and practical condition of tissues can be checked with a time resolution of 1–4 s in intact plants, without expelling them from the habitat.