Effect of Jiaweiwumei decoction on regulatory T cells and interleukin-10 in a rat model of ulcerative colitis

• Published in: 中医杂志：英文版 no. 3; pp. 312 - 315
• Main Author: Tao Mei Wang Xuyan Wang Aiqin Gao Ninghan Pan Jie Liu Xiaoquan Wang Xiaoye
• Format: Journal Article
• Language: English
• Published: 15.06.2015
• Subjects: IL-10; T淋巴细胞; 大鼠; 模型组; 溃疡性结肠炎; 白细胞介素-10; 调节性T细胞; 酶联免疫吸附法
• ISSN: 0255-2922

OBJECTIVE：To investigate the effect of a decoction made from the Traditional Chinese Medicine Wumei pill,on regulatory T cells and interleukin-10（IL-10） in a rat model of ulcerative colitis induced by2,4,6-trinitrobenzene sulfonic acid（TNBS）.METHODS：Rat ulcerative colitis was induced with TNBS.All modeled rats were randomly divided into six groups：normal control group;model group;sulfasalazine suppositories treatment group;and high,moderate,and low dosage of Jiaweiwumei decoction groups（12 rats each）.Colon injury index was evaluated after 14 days.After peripheral blood lymphocyte separation,CD4 ＋ T cells and CD4＋/CD25＋ T cell percentage was detected by flow cytometry.The content of IL-10 in serum and intestinal mucosa tissue was detected by sandwich enzyme-linked immunosorbent assay.RESULTS：Colon injury indices in the decoction groups were effectively reduced,compared with the model group（P 〈 0.05）.Compared with that of the control group,the CD4＋/CD25＋ to CD4＋ T lymphocyte ratio of the model group was significantly lower,while the decoction treatment improved the CD4 ＋/CD25 ＋ to CD4 ＋ T lymphocyte ratio（P 〈0.05）.The serum and mucosal IL-10 content of the model group was significantly lower（P 〈 0.05） than that in the control group,while the decoction group had significantly higher serum and intestinal mucosal IL-10 content than that in the model group（P 〈 0.05）.The regulatory T cell content was negatively correlated with the colonic injury index（r = 0.68,P 〈 0.05）,and positively correlated with the content of serum IL-10（r = 0.87,P 〈 0.05） and intestinal mucosal IL-10（r= 0.79,P 〈 0.05）.CONCLUSION：Jiaweiwumei decoction had significant effects on regulatory T cells and IL-10 in rats with TNBS-induced ulcerative colitis.