Quantitative Determination of Nitrogen Content in Cucumber Leaves Using Raman Spectroscopy and Multidimensional Feature Selection

• Published in: Agronomy (Basel) Vol. 15; no. 8; p. 1884
• Main Authors: Hou, Zhaolong, Tan, Feng, Li, Manshu, Gao, Jiaxin, Su, Chunjie, Jiao, Feng, Wang, Yaxuan, Zheng, Xin
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 04.08.2025
• Subjects: Accuracy; Adaptive sampling; Agriculture; Analytical chemistry; Chlorophyll; Cucumbers; Data processing; Efficiency; Enzymes; Feature extraction; Lasers; Least squares method; Leaves; Methods; Nitrates; Nitrogen; Nondestructive testing; Nutrients; Phosphorus; Physiology; Polynomials; Potassium; Precision agriculture; Prediction models; Quantitative analysis; Raman spectroscopy; Spectroscopy; Spectrum analysis; Vegetables
• ISSN: 2073-4395; 2073-4395
• DOI: 10.3390/agronomy15081884

Cucumber, a high-yielding crop commonly grown in facility environments, is particularly susceptible to nitrogen (N) deficiency due to its rapid growth and high nutrient demand. This study used cucumber as its experimental subject and established a spectral dataset of leaves under four nutritional conditions, normal supply, nitrogen deficiency, phosphorus deficiency, and potassium deficiency, aiming to develop an efficient and robust method for quantifying N in cucumber leaves using Raman spectroscopy (RS). Spectral data were preprocessed using three baseline correction methods—BaselineWavelet (BW), Iteratively Improve the Moving Average (IIMA), and Iterative Polynomial Fitting (IPF)—and key spectral variables were selected using 4-Dimensional Feature Extraction (4DFE) and Competitive Adaptive Reweighted Sampling (CARS). These selected features were then used to develop a N content prediction model based on Partial Least Squares Regression (PLSR). The results indicated that baseline correction significantly enhanced model performance, with three methods outperforming unprocessed spectra. A further analysis showed that the combination of IPF, 4DFE, and CARS achieved optimal PLSR model performance, achieving determination coefficients (R2) of 0.947 and 0.847 for the calibration and prediction sets, respectively. The corresponding root mean square errors (RMSEC and RMSEP) were 0.250 and 0.368, while the residual predictive deviation (RPDC and RPDP) values reached 4.335 and 2.555. These findings confirm the feasibility of integrating RS with advanced data processing for rapid, non-destructive nitrogen assessment in cucumber leaves, offering a valuable tool for nutrient monitoring in precision agriculture.