Tribulus terrestris Suppresses the Lipopolysaccharide-Induced Inflammatory Reaction in RAW264.7 Macrophages through Heme Oxygenase-1 Expressions

• Published in: Korean Journal of Oriental Physiology & Pathology Vol. 28; no. 1; pp. 63 - 68
• Main Author: Kim, Jai Eun
• Format: Journal Article
• Language: English
• Published: 한의병리학회 28.02.2014
• Subjects: 한의학; Tribulus terrestris L; Nitric oxide synthase; Heme Oxygenase-1; Cyclooxygenase; Nuclear factor E2-related factor 2
• ISSN: 1738-7698; 2288-2529
• DOI: 10.15188/kjopp.2014.02.28.1.63

The fruit of Tribulus terrestris L. (Zygophyllaceae) is an important source of traditional Korean and Chinese medicines. In this study, NNMBS223, consisting of the ethanol extract of T. terrestris, showed potent anti-inflammatory activities in RAW264.7 macrophages. We investigated the effect of NNMBS223 in suppressing the protein expression of inducible nitric oxide synthase (iNOS), cyclooxygenase (COX)-2 and production of iNOS-derived nitric oxide (NO), COX-2-derived prostaglandin E2 (PGE2) in lipopolysaccharide (LPS)-stimulated macrophages. In addition, NNMBS223 induced expression of heme oxygenase (HO)-1 through nuclear translocation of nuclear factor E2-related factor 2 (Nrf2) in macrophages. The effects of NNMBS223 on LPS-induced production of NO and PGE2 were partially reversed by the HO activity inhibitor tin protoporphyrin (SnPP). These findings suggest that Nrf2-dependent increases in expression of HO-1 induced by NNMBS223 conferred anti-inflammatory activities in LPS stimulated RAW264.7 macrophages. KCI Citation Count: 1