Induction and optimization of cellulases using various agro-wastes by Trichoderma virdii: Effect of alkali pretreatment

This study presents optimization of various lignocellulosics and alkali pretreatment for maximum cellulase production by Trichoderma virdii sp. Maximum endoglucanase (642 IU/L) and exoglucanase (187IU/L) activity was achieved with maize straw at 5% concentration. Oat hay was the most suitable agro-w...

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Bibliographic Details
Published inAfrican journal of biotechnology Vol. 13; no. 33; pp. 3426 - 3432
Main Authors Meenakshi, Goyal, Giridhar, Soni
Format Journal Article
LanguageEnglish
Published 13.08.2014
Subjects
Trichoderma
Zea mays
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Summary:This study presents optimization of various lignocellulosics and alkali pretreatment for maximum cellulase production by Trichoderma virdii sp. Maximum endoglucanase (642 IU/L) and exoglucanase (187IU/L) activity was achieved with maize straw at 5% concentration. Oat hay was the most suitable agro-waste for beta - glucosidase (7100 IUL super(-1)) production followed by maize straw (6500 IUL super(-1)). Maize straw was chosen in an effort to enhance cellulase production with 0.1 N NaOH, 0.5 N NaOH and I.0 N NaOH pretreatment. 0.1 N NaOH produced desirable results showing 2.5, 1.6, and 1.7 fold increase in endoglucanase, exoglucanase and beta -glucosidase activity. This 0.1 N pretreated straw produced reducing sugars 3.5 times more than untreated straw.
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ISSN:1684-5315
1684-5315
DOI:10.5897/AJB2014.13656