Optimization of carotenoids extraction from Penaeus semisulcatus shrimp wastes

• Published in: Journal of coastal life medicine Vol. 1; no. 2; pp. 95 - 97
• Main Authors: Khaniki, Gholamreza jahed, Sadighara, Parisa, Nodehi, Ramin Nabizadeh, Alimohammadi, Mahmood, Saatloo, Naiema Vakili
• Format: Journal Article
• Language: English
• Published: 28.09.2013
• Subjects: Marine; Penaeus semisulcatus; ISW, Iran
• ISSN: 2309-5288; 2309-6152
• DOI: 10.12980/JCLM.1.2013C812

Objective: To find effective method for carotenoids extraction from shrimp waste which is one of the important sources of natural carotenoids and produced in large quantities in Iran. Methods: Two methods of carotenoids extraction, enzymatic and alkaline (NaOH 1 normal) treatment, were assayed. About 5 g of gritted shrimp wastes were used at each stage. For alkaline treatment, sodium hydroxide were added to shrimp waste. After 48 h, the mixture was filtered and centrifuged. Results: Alcalase extraction produced (234.00+ or -2.00) mg/L carotenoid and NaOH extraction produced (170.00+ or -1.53) mg/L carotenoid. Based on the samples analyzed, alcalase enzyme showed more efficiency than NaOH extraction to achieve carotenoids from shrimp waste. Conclusions: It can be concluded that using alcalase enzyme for carotenoids extraction can produce higher carotenoids concentration than NaOH extraction method. So alcalase enzyme method can be used for achieving this kind of antioxidant.