Production of pectinase by Bacillus subtilis EFRL 01 in a date syrup medium
A newly isolated Bacillus subtilis EFRL 01 was used to produce pectinases in batch fermentations on various inexpensive media. A mineral medium based on waste date syrup (15 g/L) as the carbon source and yeast extract (7.5 g/L) as the nitrogen source proved to be the most effective. A 48 h batch fer...
Saved in:
Published in | African journal of biotechnology Vol. 11; no. 62; pp. 12563 - 12570 |
---|---|
Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
02.08.2012
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | A newly isolated Bacillus subtilis EFRL 01 was used to produce pectinases in batch fermentations on various inexpensive media. A mineral medium based on waste date syrup (15 g/L) as the carbon source and yeast extract (7.5 g/L) as the nitrogen source proved to be the most effective. A 48 h batch fermentation in this medium with a starting pH of 8.0 produced a pectinase titer of similar to 2,700 U/mL at the optimal fermentation temperature of 45 degree C. The optimal temperature, initial pH, the carbon source and its concentration, and the nitrogen source and its concentration, were identified after evaluation of multiple nutrient sources and fermentation conditions. |
---|---|
Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1684-5315 1684-5315 |
DOI: | 10.5897/AJB11.4236 |