Production of pectinase by Bacillus subtilis EFRL 01 in a date syrup medium

A newly isolated Bacillus subtilis EFRL 01 was used to produce pectinases in batch fermentations on various inexpensive media. A mineral medium based on waste date syrup (15 g/L) as the carbon source and yeast extract (7.5 g/L) as the nitrogen source proved to be the most effective. A 48 h batch fer...

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Published inAfrican journal of biotechnology Vol. 11; no. 62; pp. 12563 - 12570
Main Authors Qureshi, A S, Bhutto, MA, Chisti, Y, Khushk, I, Dahot, MU, Bano, S
Format Journal Article
LanguageEnglish
Published 02.08.2012
Subjects
Bacillus subtilis
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Summary:A newly isolated Bacillus subtilis EFRL 01 was used to produce pectinases in batch fermentations on various inexpensive media. A mineral medium based on waste date syrup (15 g/L) as the carbon source and yeast extract (7.5 g/L) as the nitrogen source proved to be the most effective. A 48 h batch fermentation in this medium with a starting pH of 8.0 produced a pectinase titer of similar to 2,700 U/mL at the optimal fermentation temperature of 45 degree C. The optimal temperature, initial pH, the carbon source and its concentration, and the nitrogen source and its concentration, were identified after evaluation of multiple nutrient sources and fermentation conditions.
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ISSN:1684-5315
1684-5315
DOI:10.5897/AJB11.4236