Nonenzymatically oxidized arachidonic acid regulates T-type Ca 2+ currents in mouse spermatogenic cells

During spermatogenesis, fatty acids play an important role both as structural components and messengers that trigger male germ cell line differentiation. The spontaneous oxidation of fatty acids causes a decrease in mammalian fertility. Here, we examine the effects of nonenzymatically oxidized arach...

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Bibliographic Details
Published inFEBS letters Vol. 593; no. 14; pp. 1735 - 1750
Main Authors Bondarenko, Olga, Corzo, Gerardo, Santana, Félix L, Del Río-Portilla, Federico, Darszon, Alberto, López-González, Ignacio
Format Journal Article
LanguageEnglish
Published England 01.07.2019
Subjects
arachidonic acid
oxidized fatty acids
spermatogenic cells
T-type Ca2+ channels
developmental biology
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Summary:During spermatogenesis, fatty acids play an important role both as structural components and messengers that trigger male germ cell line differentiation. The spontaneous oxidation of fatty acids causes a decrease in mammalian fertility. Here, we examine the effects of nonenzymatically oxidized arachidonic acid (AA ) on mouse spermatogenic T-type Ca currents (I ) due to their physiological relevance during spermatogenesis. AA is 25-fold more potent than AA at inhibiting I and it left shifts the I-V curve peak and both activation and steady-state inactivation curves. In addition, I deactivation kinetics and their recovery from inactivation are slower in the presence of AA . Therefore, the fraction of inactivated Ca channels is increased. AA -induced I inhibition could contribute to male infertility affecting Ca regulation in spermatogenic cells.
ISSN:0014-5793
1873-3468
DOI:10.1002/1873-3468.13448