Computer assisted semen analysis of deccani ram semen presevability at 5ºC

To investigate the preservability of Deccani ram semen in three different extenders - SC (Sodium Citrate), T (Tris), C (Coconut milk) egg yolk extenders after dilution and chilling (5ºC) at 1, 24 and 48 hr. of storage. Eight Deccani adult rams (aged 2-4 years) were selected and ejaculate from each r...

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Bibliographic Details
Published inIndian journal of animal research
Main Authors Rajashri, M, Reddy, Ramchandra, Kumari, G. Aruna, Kumari, N. Nalini, Srinivas, G.
Format Journal Article
LanguageEnglish
Published 26.04.2018
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Summary:To investigate the preservability of Deccani ram semen in three different extenders - SC (Sodium Citrate), T (Tris), C (Coconut milk) egg yolk extenders after dilution and chilling (5ºC) at 1, 24 and 48 hr. of storage. Eight Deccani adult rams (aged 2-4 years) were selected and ejaculate from each ram was divided into 3 aliquots and diluted with SC, T and C extenders (final concentration - 400 million spermatozoa/0.2 ml semen) and stored at 5ºC. Semen evaluation was done by computer assisted semen analyser (CASA). Analysis of Variance (ANOVA) was used to test the significance difference between extenders. S: Sodium citrate (SC) and Tris (T) - Egg yolk extender was significantly (P less than 0.05) better as compared to Coconut milk extender (C). No significant difference (P>0.05) was observed between SC and T. Sperm kinematics assessment by CASA revealed Sodium citrate-based egg-yolk extender (SC) as better extender for preservation of Deccani Ram semen upto 48 hr. of storage as compared to T and C.
ISSN:0367-6722
0976-0555
DOI:10.18805/ijar.B-3450