Controllable Preparation of 2D V 2 O 5 Peroxidase-Mimetic Nanozyme to Develop Portable Paper-Based Analytical Device for Intelligent Pesticide Assay

• Published in: Small (Weinheim an der Bergstrasse, Germany) Vol. 19; no. 14; p. e2206465
• Main Authors: Li, Haiyin, Zhao, Suixin, Wang, Zhixin, Li, Feng
• Format: Journal Article
• Language: English
• Published: Germany 01.04.2023
• Subjects: Coloring Agents; Oxidation-Reduction; Peroxidase; Peroxidases; Pesticides; paper-based analytical devices; naked-eye and portable analysis; peroxidase-mimetic nanozymes; π-π stacking; pesticides
• ISSN: 1613-6810; 1613-6829
• DOI: 10.1002/smll.202206465

Given severe harmfulness of pesticides, unique characteristics of peroxidase-mimetic nanozymes, and favorable prospects of paper-based analytical devices (PADs), it is highly desirable to construct a nanozyme-based PAD for intelligent analysis of pesticide without enzyme/aptamer/antibody and interference of O . Herein, 2D nanosheet-like V O (2D-VONz) with exclusive peroxidase-mimetic activity is controllably prepared under the optimal reactants concentration and reaction temperature. Experimental characterizations demonstrate that 2D-VONz exhibits high affinity and catalytic rate, and catalytic oxidation is dependent on •OH yielded from the decomposition of H O catalyzed by 2D-VONz, and the catalytic performance is relevant to π-π stacking force-controlled surface zeta potential of 2D-VONz changed by substrates, giving a comprehensive understand of the inherent mechanism. Interestingly, 2D-VONz activity is inhibited by pesticide glyphosate (Gly), and then is exploited to develop a PAD, on which, Gly declines 2D-VONz activity to prevent it from catalyzing the oxidation of 3,3',5,5'-tetramethylbenzidine, contributing to rapid, naked-eye, and portable analysis of pesticide using a smartphone. The current strategy on preparing exclusive peroxidase-mimetic 2D nanozyme, investigating catalytic mechanism, developing nanozyme-based PAD, and achieving direct pesticide sensing will set up new avenues to improve the analytical performance, strengthen the practicability, and broaden the application scope of nanozymes.