Controllable Preparation of 2D V 2 O 5 Peroxidase-Mimetic Nanozyme to Develop Portable Paper-Based Analytical Device for Intelligent Pesticide Assay
Given severe harmfulness of pesticides, unique characteristics of peroxidase-mimetic nanozymes, and favorable prospects of paper-based analytical devices (PADs), it is highly desirable to construct a nanozyme-based PAD for intelligent analysis of pesticide without enzyme/aptamer/antibody and interfe...
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Published in | Small (Weinheim an der Bergstrasse, Germany) Vol. 19; no. 14; p. e2206465 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Germany
01.04.2023
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Subjects | |
Online Access | Get full text |
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Summary: | Given severe harmfulness of pesticides, unique characteristics of peroxidase-mimetic nanozymes, and favorable prospects of paper-based analytical devices (PADs), it is highly desirable to construct a nanozyme-based PAD for intelligent analysis of pesticide without enzyme/aptamer/antibody and interference of O
. Herein, 2D nanosheet-like V
O
(2D-VONz) with exclusive peroxidase-mimetic activity is controllably prepared under the optimal reactants concentration and reaction temperature. Experimental characterizations demonstrate that 2D-VONz exhibits high affinity and catalytic rate, and catalytic oxidation is dependent on •OH yielded from the decomposition of H
O
catalyzed by 2D-VONz, and the catalytic performance is relevant to π-π stacking force-controlled surface zeta potential of 2D-VONz changed by substrates, giving a comprehensive understand of the inherent mechanism. Interestingly, 2D-VONz activity is inhibited by pesticide glyphosate (Gly), and then is exploited to develop a PAD, on which, Gly declines 2D-VONz activity to prevent it from catalyzing the oxidation of 3,3',5,5'-tetramethylbenzidine, contributing to rapid, naked-eye, and portable analysis of pesticide using a smartphone. The current strategy on preparing exclusive peroxidase-mimetic 2D nanozyme, investigating catalytic mechanism, developing nanozyme-based PAD, and achieving direct pesticide sensing will set up new avenues to improve the analytical performance, strengthen the practicability, and broaden the application scope of nanozymes. |
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ISSN: | 1613-6810 1613-6829 |
DOI: | 10.1002/smll.202206465 |