Wound-Healing Material with Antibacterial and Antioxidant Functions, Constructed Using Keratin, Hyperbranched Polymers, and MnO 2

• Published in: ACS applied materials & interfaces Vol. 15; no. 25; pp. 29841 - 29853
• Main Authors: Lu, Yongping, Ye, Wenjin, Kang, Weiqi, Wang, Shuaibing, Zhu, Zhongqiang, Chen, Xingyu, Li, Jianshu
• Format: Journal Article
• Language: English
• Published: United States 28.06.2023
• Subjects: keratin; wound healing; antibacterial; hydrogel; hyperbranched polymer
• ISSN: 1944-8244; 1944-8252
• DOI: 10.1021/acsami.3c03237

Wound healing, a global medical issue, poses a substantial financial burden. Therefore, developing low-cost and highly efficacious wound-healing materials is essential. In this study, we prepared keratin-hyperbranched polymer hydrogel-M (KHBP-M), a multifunctional composite gel, by mixing reduced keratin containing free sulfhydryl groups extracted from human hair waste, hyperbranched polymer (HBP) with double bonds at the end, and MnO nanoparticles prepared using the biological template method. Keratin has intrinsic wound-healing properties, and MnO is a wound-healing material with both photothermal antibacterial and reactive oxygen species (ROS)-scavenging abilities. KHBP-M showed antibacterial effects against both Gram-positive ( ) and Gram-negative ( ) bacteria. When exposed to irradiation (808 nm), the killing ratio for reached 99.99%, which is especially suitable for wound environments. A similar trend was noted for . The composite hydrogel also showed excellent ROS-scavenging ability and could resist oxidative stress in L929 cells. Furthermore, in an animal model of infected wounds, the KHBP-M hydrogel treated with near-infrared light had the fastest wound-healing rate, reaching 82.98% on day 15. Our study provides a promising wound-healing material, with simple preparation methods, easy access to sources, and low cost involved.