Haemoglobin Epsilon as a Biomarker for the Molecular Detection of Canine ‎Lymphoma

• Published in: The Iraqi journal of veterinary medicine Vol. 47; no. 1; pp. 21 - 27
• Main Authors: H Al-Kinani, Layla, A Sharp, Margaret, M Wyatt, Kenneth, Coiacetto, Flaminia, R Sharp, Claire, Rossi, Gabriele, K Greene, Wayne
• Format: Journal Article
• Language: English
• Published: University of Baghdad, College of Veterinary Medicine 01.07.2023
• Subjects: canine lymphoma; embryonic haemoglobin; RT-qPCR; subunit epsilon
• ISSN: 1609-5693; 2410-7409
• DOI: 10.30539/ijvm.v47i1.1494

Lymphoma is a cancer arising from B or T lymphocytes that are central immune system ‎components. It is one of the three most common cancers encountered in the canine; ‎lymphoma affects middle-aged to older dogs and usually stems from lymphatic tissues, ‎such as lymph nodes, lymphoid tissue, or spleen. Despite the advance in the management of ‎canine lymphoma, a better understanding of the subtype and tumor aggressiveness is still ‎crucial for improved clinical diagnosis to differentiate malignancy from hyperplastic ‎conditions and to improve decision-making around treating and what treatment type to use. ‎This study aimed to evaluate a potential novel biomarker related to iron metabolism, ‎embryonic haemoglobin (HBE), for early diagnosis. Archived samples in combination with ‎prospective samples collected from dogs with and without lymphoma were used in this ‎study for the retrospective analyses of this tumor based on the same biomarker amplified by ‎real-time quantitative polymerase chain reaction. The HBE mRNA was aberrantly expressed ‎in canine B and T cell lymphoma compared to the normal lymph node tissue and ‎hyperplastic lymph nodes. In conclusion, this study identified a novel potential biomarker for improving lymphoma ‎diagnosis and treatment in dogs. Further studies with larger sample sizes are needed to ‎confirm the suitability of this biomarker for canine lymphoma diagnosis.