Colony Forming Assay for HCV-Replicon Cell Line

Hepatitis C virus (HCV) is the main causative agent of chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma. Since the HCV genome is present exclusively in RNA form during replication, a number of anti-HCV drugs show appearance of rapid drug-resistant viruses. Therefore, it is important...

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Bibliographic Details
Published inBio-protocol Vol. 3; no. 24
Main Authors Chang Ho Lee, Seong-Wook Lee
Format Journal Article
LanguageEnglish
Published Bio-protocol LLC 01.12.2013
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Summary:Hepatitis C virus (HCV) is the main causative agent of chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma. Since the HCV genome is present exclusively in RNA form during replication, a number of anti-HCV drugs show appearance of rapid drug-resistant viruses. Therefore, it is important to test generation of drug-escape mutant viruses by developed antiviral drugs for their validity. Here, we describe a colony formation assay-based method to observe appearance of drug-resistant viruses against nucleic acid based anti-HCV drugs in genotype 1b based subgenomic replicon cell culture system (Lee et al., 2013).
ISSN:2331-8325
DOI:10.21769/BioProtoc.1009