The use of real time PCR for quantitative determination of some propionic bacteria residing on human skin

A test system has been developed for determination of propionic bacterial species residing on human skin. This system developed in the real time PCR format is applicable for quantitative determination and also detection of genomes of the following Propionibacterium species: P. acnes, P. granulosum a...

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Bibliographic Details
Published inBiomeditsinskaia khimiia Vol. 58; no. 5; p. 608
Main Authors Globa, A G, Alekseev, Ia I, Arzumanian, V G, Zaborova, V A, Guridov, A A
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.09.2012
Subjects
Adult
Humans
Male
Propionibacterium acnes - genetics
Propionibacterium acnes - isolation & purification
Real-Time Polymerase Chain Reaction - methods
Skin - microbiology
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Summary:A test system has been developed for determination of propionic bacterial species residing on human skin. This system developed in the real time PCR format is applicable for quantitative determination and also detection of genomes of the following Propionibacterium species: P. acnes, P. granulosum and P. avidum. This system was used for analysis of wash samples from the skin of 17 pentathlon sportsmen and 16 students. All three species of propionic bacteria were found in all skin wash samples. However, contamination with P. acnes was two times higher in control group than in the group of pentathlon sportsmen.
ISSN:2310-6972
DOI:10.18097/pbmc20125805608