1α,25-dihydroxyvitamin D 3 -eluting nanofibrous dressings induce endogenous antimicrobial peptide expression

• Published in: Nanomedicine (London, England) Vol. 13; no. 12; pp. 1417 - 1432
• Main Authors: Jiang, Jiang, Zhang, Yang, Indra, Arup K, Ganguli-Indra, Gitali, Le, Mai N, Wang, Hongjun, Hollins, Ronald R, Reilly, Debra A, Carlson, Mark A, Gallo, Richard L, Gombart, Adrian F, Xie, Jingwei
• Format: Journal Article
• Language: English
• Published: England 01.06.2018
• Subjects: Animals; Anti-Infective Agents - administration & dosage; Anti-Infective Agents - chemistry; Antimicrobial Cationic Peptides - chemistry; Antimicrobial Cationic Peptides - metabolism; Bandages - microbiology; Cell Line; Humans; Keratinocytes - drug effects; Keratinocytes - microbiology; Mice; Mice, Transgenic; Monocytes - drug effects; Nanofibers - administration & dosage; Nanofibers - chemistry; Vitamin D - administration & dosage; Vitamin D - analogs & derivatives; Vitamin D - chemistry; Wound Closure Techniques; 1α,25-dihydroxyvitamin D3; antimicrobial peptides LL-37; endogenous production; electrospun nanofibers; innate immunity; sustained delivery
• ISSN: 1743-5889; 1748-6963
• DOI: 10.2217/nnm-2018-0011

The aim of this study was to develop a nanofiber-based dressing capable of local sustained delivery of 1α,25-dihydroxyvitamin D (1,25(OH) D ) and augmenting human CAMP induction. Nanofibrous wound dressings containing 1,25(OH) D were successfully prepared by electrospinning, which were examined in vitro, in vivo and ex vivo. 1,25(OH) D was successfully loaded into nanofibers with encapsulation efficiency larger than 90%. 1,25(OH) D showed a sustained release from nanofibers over 4 weeks. Treatment of U937 and HaCaT cells with 1,25(OH) D -loaded poly(ϵ-caprolactone) nanofibers significantly induced hCAP18/LL37 expression in monocytes and keratinocytes, skin wounds of humanized transgenic mice and artificial wounds of human skin explants. 1,25(OH) D containing nanofibrous dressings could enhance innate immunity by inducing antimicrobial peptide production.