Artemisia dracunculus Extracts Obtained by Organic Solvents and Supercritical CO 2 Produce Cytotoxic and Antitumor Effects in Mice with L5178Y Lymphoma

• Published in: Journal of medicinal food Vol. 20; no. 11; p. 1076
• Main Authors: Navarro-Salcedo, Martha Hilda, Delgado-Saucedo, Jorge Ivan, Siordia-Sánchez, Victor Hugo, González-Ortiz, Luis J, Castillo-Herrera, Gustavo Adolfo, Puebla-Pérez, Ana M
• Format: Journal Article
• Language: English
• Published: United States 01.11.2017
• Subjects: Animals; Antineoplastic Agents, Phytogenic - administration & dosage; Antineoplastic Agents, Phytogenic - isolation & purification; Artemisia - chemistry; Cell Line, Tumor; Chromatography, Supercritical Fluid; Humans; Lymphoma - drug therapy; Male; Mice; Mice, Inbred BALB C; Plant Extracts - administration & dosage; Plant Extracts - isolation & purification; Polyphenols - administration & dosage; Polyphenols - isolation & purification; extract organic solvent; Artemisia dracunculus; antitumor activity; extract supercritical CO2
• ISSN: 1557-7600
• DOI: 10.1089/jmf.2017.0044

We investigated the cytotoxic and antitumor effects of nine leaf extracts from Artemisia dracunculus (Tarragon). Five extracts were obtained using different organic solvents and four by supercritical CO . The cytotoxic effects were expressed as IC in 100, 80, 80, 100, and 80 μg/mL by respective solvents: hexane, ethyl acetate, acetone, ethanol, and acetonitrile in L5178Y lymphoma cells. For supercritical CO extract A, IC was 100 μg/mL; for extracts C and D, IC was 150 μg/mL. The antitumor activity was assessed through a tumor growth inhibition test that measured ascites fluid volume and tumor cell counts of BALB/c mice (2 × 10 cells L5178Y i.p.). Twenty-four hours after inoculation, mice were treated with 100 mg/kg of acetonitrile extract or extract SF-A daily for 15 days in independent groups of five mice, using two administration routes. We observed tumor evolution with and without treatment. Without treatment, tumor evolution was 17,969 × 10  ± 5485 L5178Y cells in 2.6 mL ascites volume, whereas the orally treated acetonitrile extract group showed 0.1 × 10  ± 0.07 L5178Y cells (P < .05). The oral SF-A group showed 12.9 × 10  ± 243 L5178Y cells, and intraperitoneal (i.p.)-treated SF-A group showed 0.1 × 10  ± 0.05 L5178Y cells (P < .05) without any ascites volume development. The acetonitrile extract contains abundant polyphenols and possibly a flavone with antioxidant activity. The SF-A contains abundant alkamides. Both extracts are complexes and the identity of the compounds responsible for observed antitumor activity remains unknown.