Cloning and Expression of the Anti tumor Glycoprotein Gene of Streptococcus pyogenes Su in Escherichia coli

The structural gene for the streptococcal acid glycoprotein (SAGP), an antitumor glycoprotein produced by Streptococcus pyogenes Su, was cloned in Escherichia coli, and the nucleotide sequence was determined. The deduced amino acid sequence of SAGP was composed of 410 amino acids, and was highly hom...

Full description

Saved in:
Bibliographic Details
Published inAgricultural and biological chemistry Vol. 51; no. 10; pp. 2641 - 2648
Main Authors Kanaoka, Masaharu, Kawanaka, Chigusa, Negoro, Takaharu, Fukita, Yoshikazu, Taya, Kiyoshi, Agui, Hideo
Format Journal Article
LanguageEnglish
Published Taylor & Francis 01.10.1987
Online AccessGet full text

Cover

More Information
Summary:The structural gene for the streptococcal acid glycoprotein (SAGP), an antitumor glycoprotein produced by Streptococcus pyogenes Su, was cloned in Escherichia coli, and the nucleotide sequence was determined. The deduced amino acid sequence of SAGP was composed of 410 amino acids, and was highly homologous with colicin E1, a bacterial antimicrobial protein. The SAGP gene was subcloned into an expression plasmid and was expressed in E. coli. The gene product was demonstrated to have almost the same molecular weight and an immuno-chemical property with those of the native SAGP.
ISSN:0002-1369
DOI:10.1080/00021369.1987.10868453