Coexistence of Fosfomycin Resistance Determinant fosA and fosA 3 in Enterobacter cloacae Isolated from Pets with Urinary Tract Infection in Taiwan

• Published in: Microbial drug resistance (Larchmont, N.Y.) Vol. 27; no. 3; p. 415
• Main Authors: Chen, Lin, Ou, Bingming, Zhang, Minyu, Chou, Chung-Hsi, Chang, Shao-Kuang, Zhu, Guoqiang
• Format: Journal Article
• Language: English
• Published: United States 01.03.2021
• Subjects: companion pets; fosfomycin-resistant determinant; fosA3; genetic environment; fosA
• ISSN: 1931-8448
• DOI: 10.1089/mdr.2020.0077

To analyze the characteristics of and 3 in isolated from aspirated and catheterized urine culture specimens of companion pets in Taiwan. A total of 19 isolates from pets with urinary tract infection were screened for the presence of , 3, and and for the genetic context of them by PCR amplification and sequencing. The transferability, resistance phenotypes, plasmid replicon typing properties and genetic environments of - and/or 3-positive strains were characterized. Five isolates were positive for and three coharbored and 3. No determinant was detected. Transconjugants of 3 were successfully acquired, while the acquisition of transconjugants was failed. The minimum inhibitory concentrations (MICs) of the three 3-positive isolates and their transconjugants were ≥256 mg/L, whereas the MICs of the five -positive isolates ranged from 64 mg/L to 256 mg/L. Three plasmid replicons ( FrepB, L/M, and HI2) were identified in - and -positive isolates. Different genetic contexts lay in the downstream region of and 3, respectively. Eight distinct patterns based on the similarity value of more than 80% were typed for all the 8 -positive isolates. In conclusion, the concomitant with 3 were found in isolates. The 3 not only exhibits stronger activity of inactivating fosfomycin than but also possesses stronger potential to spread than . Different genetic backgrounds exist in these - and -positive isolates, and different mobile elements may confer the dissemination of and .