Collection of Peripheral Blood CD 34+ Progenitor Cells from Healthy Dogs and Dogs Diagnosed with Lymphoproliferative Diseases Using a B axter‐ F enwal CS ‐3000 Plus Blood Cell Separator

• Published in: Journal of veterinary internal medicine Vol. 25; no. 6; pp. 1406 - 1413
• Main Author: Suter, S.E.
• Format: Journal Article
• Language: English
• Published: 01.11.2011
• ISSN: 0891-6640; 1939-1676
• DOI: 10.1111/j.1939-1676.2011.00827.x

Background Canine peripheral blood mononuclear cell ( PBMC ) apheresis using a B axter‐ F enwal CS ‐3000 Plus automated blood cell separator has not been reported. Objective To determine the feasibility and safety of using a CS ‐3000 Plus blood cell separator with a small volume separation container holder ( SVSCH ) and small volume collection chamber ( SVCC ) to harvest canine PBMC s from dogs weighing <50 kg. Animals Eight healthy mongrel dogs and 11 client‐owned dogs in clinical remission for lymphoproliferative diseases ( LPD ). Methods In this prospective study, aphereses were performed using a B axter‐ F enwal CS ‐3000 Plus blood cell separator, with or without recombinant human granulocyte colony‐stimulating factor (rh G ‐ CSF ) treatment. Results Aphereses from 6 healthy dogs given rh G ‐ CSF yielded an average of 1.1 × 10 7  ± 8.2 × 10 6 CD 34+ cells/kg. Aphereses from LPD dogs given rh G ‐ CSF yielded an average of 5.4 × 10 6  ± 3.25 × 10 6 CD 34+ cells/kg ( P  = .17). Higher hematocrit in both groups of dogs receiving rh G ‐ CSF correlated with an increased number of CD 34+ cells/kg harvested (healthy, P  = .04; LPD , P  = .05). Apheresis was well tolerated by all dogs. Conclusions and Clinical Importance Canine PBMC apheresis using the B axter‐ F enwal CS ‐3000 Plus cell separator with an SVSCH and SVCC is a feasible and safe option for harvesting an adequate number of CD 34+ peripheral blood progenitor cells from dogs weighing ≥17 kg for hematopoietic cell transplantation.