Collection of Peripheral Blood CD 34+ Progenitor Cells from Healthy Dogs and Dogs Diagnosed with Lymphoproliferative Diseases Using a B axter‐ F enwal CS ‐3000 Plus Blood Cell Separator
Background Canine peripheral blood mononuclear cell ( PBMC ) apheresis using a B axter‐ F enwal CS ‐3000 Plus automated blood cell separator has not been reported. Objective To determine the feasibility and safety of using a CS ‐3000 Plus blood cell separator with a small volume separation container...
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Published in | Journal of veterinary internal medicine Vol. 25; no. 6; pp. 1406 - 1413 |
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Main Author | |
Format | Journal Article |
Language | English |
Published |
01.11.2011
|
Online Access | Get full text |
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Summary: | Background
Canine peripheral blood mononuclear cell (
PBMC
) apheresis using a
B
axter‐
F
enwal
CS
‐3000
Plus
automated blood cell separator has not been reported.
Objective
To determine the feasibility and safety of using a
CS
‐3000
Plus
blood cell separator with a small volume separation container holder (
SVSCH
) and small volume collection chamber (
SVCC
) to harvest canine
PBMC
s from dogs weighing <50 kg.
Animals
Eight healthy mongrel dogs and 11 client‐owned dogs in clinical remission for lymphoproliferative diseases (
LPD
).
Methods
In this prospective study, aphereses were performed using a
B
axter‐
F
enwal
CS
‐3000
Plus
blood cell separator, with or without recombinant human granulocyte colony‐stimulating factor (rh
G
‐
CSF
) treatment.
Results
Aphereses from 6 healthy dogs given rh
G
‐
CSF
yielded an average of 1.1 × 10
7
± 8.2 × 10
6
CD
34+ cells/kg. Aphereses from
LPD
dogs given rh
G
‐
CSF
yielded an average of 5.4 × 10
6
± 3.25 × 10
6
CD
34+ cells/kg (
P
= .17). Higher hematocrit in both groups of dogs receiving rh
G
‐
CSF
correlated with an increased number of
CD
34+ cells/kg harvested (healthy,
P
= .04;
LPD
,
P
= .05). Apheresis was well tolerated by all dogs.
Conclusions and Clinical Importance
Canine
PBMC
apheresis using the
B
axter‐
F
enwal
CS
‐3000
Plus
cell separator with an
SVSCH
and
SVCC
is a feasible and safe option for harvesting an adequate number of
CD
34+ peripheral blood progenitor cells from dogs weighing ≥17 kg for hematopoietic cell transplantation. |
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ISSN: | 0891-6640 1939-1676 |
DOI: | 10.1111/j.1939-1676.2011.00827.x |