Rapid diagnostic method for discriminating two types of COI sequences in the gall‐forming aphid T etraneura nigriabdominalis ( H emiptera: A phididae) by multiplex polymerase chain reaction
Abstract T etraneura nigriabdominalis ( S asaki, 1899) is a galling aphid that is distributed widely in E urasia. In the present study, a diagnostic multiplex polymerase chain reaction ( PCR ) protocol was developed to discriminate two types of the cytochrome oxidase subunit I ( COI ) sequences in t...
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| Published in | Entomological science Vol. 16; no. 2; pp. 243 - 247 |
|---|---|
| Main Authors | , , |
| Format | Journal Article |
| Language | English |
| Published |
01.04.2013
|
| Online Access | Get full text |
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| Summary: | Abstract
T
etraneura nigriabdominalis
(
S
asaki, 1899) is a galling aphid that is distributed widely in
E
urasia. In the present study, a diagnostic multiplex polymerase chain reaction (
PCR
) protocol was developed to discriminate two types of the cytochrome oxidase subunit I (
COI
) sequences in this species, and its usefulness was examined from 25 samples of
T
. nigriabdominalis
. Multiplex
PCR
and electrophoresis revealed that 10 and 15 samples had one band of 133 bp (type
A
) and 426 bp (type
B
), respectively. This discrimination was identical to the division of the 25 samples based on the alignments of
COI
sequences (511 bp), indicating high accuracy of this protocol in distinguishing the two types. This protocol is useful for studying microgeographic distributions of the two types and genetic diversities in
T
. nigriabdominalis
. |
|---|---|
| ISSN: | 1343-8786 1479-8298 |
| DOI: | 10.1111/j.1479-8298.2012.00557.x |