RNA analysis with a novel fluorescent oligonucleotide

We have designed and synthesized a novel fluorescent oligonucleotide to analyze functions of RNAs. This probe worked as a green fluorophore only when making a stable doublestrand with DNA or RNA. The fluorescence enhancement is rapid, large and target specific. Therefore, this probe has an ideal pho...

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Bibliographic Details
Published inNucleic Acids Symposium Series Vol. 51; no. 1; pp. 291 - 292
Main Authors Kubota, Takeshi, Ikeda, Shuji, Okamoto, Akimitsu
Format Journal Article
LanguageEnglish
Published Oxford University Press 01.11.2007
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Summary:We have designed and synthesized a novel fluorescent oligonucleotide to analyze functions of RNAs. This probe worked as a green fluorophore only when making a stable doublestrand with DNA or RNA. The fluorescence enhancement is rapid, large and target specific. Therefore, this probe has an ideal photophysical property as a fluorescent probe. We applied a fluorescent oligonucleotide to living cells to confirm the condition for introducing the probe. This novel probe and analyzing method will be a very useful tool for understanding RNA functions.
ISSN:0261-3166
1746-8272
DOI:10.1093/nass/nrm146