Properties and EPR studies of oligodeoxynucleotides containing 2-N-tert-butylaminoxylpurines

Oligodeoxynucleotide (ODN; 1) containing 2-N-tert-butylaminoxyladenosine (2) was synthesized by the conventional phosphoramidite method. The thermal stability and structure of the duplex of ODN 1 and the complementary strand were elucidated by UV-melting temperature measurements and CD experiments....

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Bibliographic Details
Published inNucleic Acids Symposium Series Vol. 49; no. 1; pp. 3 - 4
Main Authors Aso, Mariko, Kaneko, Toshiyuki, Mirc, John Walter, Koga, Noboru, Suemune, Hiroshi
Format Journal Article
LanguageEnglish
Published Oxford University Press 01.09.2005
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Summary:Oligodeoxynucleotide (ODN; 1) containing 2-N-tert-butylaminoxyladenosine (2) was synthesized by the conventional phosphoramidite method. The thermal stability and structure of the duplex of ODN 1 and the complementary strand were elucidated by UV-melting temperature measurements and CD experiments. In phosphate buffer, ODN 1 showed a three line EPR spectrum. Upon duplex formation, the EPR spectrum of 1 changed which indicates that EPR spectra of 1 can provide information on structures of ODN.
ISSN:0261-3166
1746-8272
DOI:10.1093/nass/49.1.3