Effects of rumen-protected amino acid prototypes on rumen fermentation characteristics in vitro

• Published in: Korean Journal of Agricultural Science Vol. 48; no. 4; pp. 669 - 679
• Main Authors: Kim, Gyeongjin, Marbun, Tabita Dameria, Park, Jinhyun, Lee, Sang Moo, Lee, Hong Gu, Moon, Jun Ok, Park, Jin Seung, Kim, Eun Joong
• Format: Journal Article
• Language: English
• Published: 충남대학교 농업과학연구소 01.12.2021; 농업과학연구소
• Subjects: 농학; fermentation; rumen-protected; amino acids; in vitro
• ISSN: 2466-2402; 2466-2410
• DOI: 10.7744/kjoas.20210056

This study was conducted to evaluate the effects of rumen-protected amino acid (RPAA) prototypes, which were chemically synthesized, on in vitro rumen fermentation and protection rate outcomes. Several RPAA prototypes were incubated with timothy hay and concentrate. Treatments consisted of 1) control (CON; no RPAA prototype supplement), and prototypes of 2) 0.5% RP-methionine (RPMet), 3) 0.5% RP-tryptophan (RPTrp), 4) 0.5% RPvaline (RPVal), 5) 0.5% RP-phenylalanine (RPPhe), 6) 0.5% RP-leucine (RPLeu), 7) 0.5% RPhistidine (RPHis), 8) 20% RPMet, and 9) 20% RPTrp (w·w-1 feed). The inoculum (50 mL) prepared with rumen fluid and McDougall’s buffer (1 : 4) was dispensed in individual serum bottles and was anaerobically incubated for 0, 6, and 24 h at 39℃ in triplicate. The dry matter degradability did not differ among the groups, except for the 20% RPMet and the 20% RPTrp treatments at 6 and 24 h. The total volatile fatty acid concentration in the 20% RPMet was higher (p < 0.05) than the rest of the groups at 6 h, and 20% RPMet showed the highest molar proportion of acetate, whereas the lowest proportion of propionate was found at 6 h (p < 0.05). The protection rate of the RPAA prototypes ranged from 29.85 to 109.21%. at 24 h. In conclusion, the chemically synthesized RPAA prototypes studied here had no detrimental effects on rumen fermentation parameters. Further studies using animal models are needed for more accurate evaluations of the effectiveness of RPAA. KCI Citation Count: 0