Occurrence of extended-spectrum β-lactamases amongEscherichia coli isolates from hospitalized and healthy children

The prevalence of extended-spectrum β-lactamases (ESBL) was determined among isolates ofEscherichia coli (n=63) isolated from hospitalized (43) and healthy (20) children. Ten isolates (21 %) were ESBL-positive for two screening tests, the double disk-synergy test and theOxoid Combination Disk method...

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Bibliographic Details
Published inFolia microbiologica Vol. 48; no. 2; pp. 243 - 247
Main Authors Franiczek, R., Sobieszczańska, B., Grabowski, M., Mowszet, K., Pytrus, T.
Format Journal Article
LanguageEnglish
Published Dordrecht Springer Nature B.V 01.03.2003
Subjects
Amides
Bacterial infections
Carbapenems
Children
Children & youth
Clavulanic acid
E coli
Enzymes
Gastrointestinal diseases
Microbiology
β Lactamase
β-Lactam antibiotics
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Summary:The prevalence of extended-spectrum β-lactamases (ESBL) was determined among isolates ofEscherichia coli (n=63) isolated from hospitalized (43) and healthy (20) children. Ten isolates (21 %) were ESBL-positive for two screening tests, the double disk-synergy test and theOxoid Combination Disk method. One ESBL-positive isolate came from a healthy child. The transfer frequency of oxyimino-β-lactam resistance from ESBL-producing isolates toE. coli K12 C600 recipient strain ranged from 10−8 to 10−5 per donor cell. Donor strains and transconjugants displayed susceptibility patterns typical of ESBL producers. They were resistant to oxyimino-β-lactams but susceptible to clavulanic acid and carbapenems. Seven out of the 10 ESBL-positive isolates were found to produce MR/MS fimbria, which may play an important role in the colonization of the human intestinal mucosa.
ISSN:0015-5632
1874-9356
DOI:10.1007/BF02930963