P073 MESENCHYMAL STEM/STROMAL CELLS REGULATE GUT INFLAMMATION AND INFECTION VIA IL-33 PRODUCTION

• Published in: Inflammatory bowel diseases Vol. 24; no. suppl_1; pp. S26 - S27
• Main Authors: Mahlakõiv, Tanel, Bryce, Paul, Artis, David
• Format: Journal Article
• Language: English
• Published: US Oxford University Press 18.01.2018
• Subjects: Crohn's disease; Cytokines; Dendritic cells; Feces; Gastroenterology; Infections; Inflammation; Inflammatory bowel disease; Kinases; Neutrophils; Permeability; Protein expression; Proteins; Stem cells
• ISSN: 1078-0998; 1536-4844
• DOI: 10.1093/ibd/izy019.081

Abstract Interleukin (IL-)33 is a cytokine that possesses dichotomous functions in the gut. Whereas various cell types have been reported to produce IL-33, conventionally it is believed to be released from damaged intestinal epithelial cells. Many cell types express the ST2 receptor complex and respond to IL-33 signal, leading to context dependent pro- or anti-inflammatory responses. We have employed antibody staining and a novel IL-33-GFP reporter mouse to characterize the IL-33-producing cell types in the gut in health and various inflammatory or infection conditions. Besides epithelial cells there are several immune and structural cell populations that express IL-33 at a high level at steady state or in disease. To address the significance of these cell types in IL-33 production, we have employed an IL-33 floxed mouse. Importantly, we show that besides epithelial cells, mesenchymal stem/stromal cells are crucial producers of IL-33 in gastrointestinal parasite infection and gut inflammation models.