Existence de “rescue” pour l'activité transformante d'un acide désoxyribonucléique après irradiation X
The damages produced by X-rays do not prevent either denaturation or renaturation of DNA. It was possible to prepare hybrid molecules with an X-ray-inactivated DNA carrying a genetic marker (streptomycin resistance) and a wild-type DNA. We have examined the rescue of transforming activity of the irr...
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| Published in | Biochimica et biophysica acta. Specialized section on nucleic acids and related subjects Vol. 91; no. 2; pp. 281 - 288 |
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| Main Author | |
| Format | Journal Article |
| Language | French |
| Published |
Elsevier B.V
01.10.1964
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| Online Access | Get full text |
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| Summary: | The damages produced by X-rays do not prevent either denaturation or renaturation of DNA. It was possible to prepare hybrid molecules with an X-ray-inactivated DNA carrying a genetic marker (streptomycin resistance) and a wild-type DNA. We have examined the rescue of transforming activity of the irradiated DNA, in respect of the irradiation dose and of the concentration ratio of the two DNA's at the time of the renaturation.
Aux cours des irradiations aux rayons X les lésions produites dans le DNA ne s'opposent ni à sa dénaturation ni à sa condensation. Il a donc été possible de former des molécules hybrides entre un DNA porteur d'un marqueur génétique (résistance à la streptomycine) inactivé aux rayons X, et un DNA de type sauvage. Nous avons étudié la restauration d'activité transformante du DNA irradié, en fonction de la dose d'irradiation et du rapport des concentrations des deux DNA au moment de la condensation. |
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| ISSN: | 0926-6550 1878-2256 |
| DOI: | 10.1016/0926-6550(64)90252-X |