A novel molecularly imprinted solid phase based on SiO2-Ag2O double-nucleation doped with graphene oxide for extraction and determination of flavonoids from Acanthaceae ebracteatus via HPLC-MS/MS

• Published in: Microchemical journal Vol. 207; p. 111900
• Main Authors: Lin, Hongling, Ma, Xingbin, Zhou, Hanlin, Shang, Xiaofei, Wang, Shengyi, Wei, Long, Li, Bing, Bai, Yubin, Wang, Weiwei, Qiu, Yanhua, Zhang, Chao, Wang, Qing, Zhou, Xuzheng, She, Yongxin, Yuan, Ligang, Zhang, Jiyu, Abd El-Aty, A.M.
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.12.2024
• Subjects: Acanthaceae ebracteatus; Determination; Extraction; Flavonoids; Molecularly imprinted polymer; Flavonoids; Extraction; Determination; Acanthaceae ebracteatus; Molecularly imprinted polymer
• ISSN: 0026-265X
• DOI: 10.1016/j.microc.2024.111900

[Display omitted] •A novel method for flavonoid extraction from marine mangrove plants was developed.•Graphene oxide with SiO2-Ag2O core was used to create multilayer surface-imprinted polymers for enhanced selectivity.•The imprinted polymer was synthesized using acacetin as a template and characterized by UV, FT-IR, SEM, XRD, and TEM techniques.•Langmuir model better predicted the adsorption compared to Freundlich.•The imprinted polymer was validated for selectivity, precision, and accuracy in flavonoid extraction from Acanthaceae ebracteatus using LC–MS/MS. (Objectives) A novel method was developed for extracting and determining flavonoids from marine mangrove medicinal plants. This approach utilizes surface molecular imprinting to increase selectivity. Graphene oxide doped with a SiO2–Ag2O dual core structure served as a scaffold for synthesizing multilayer surface-imprinted polymers. The surface of the SiO2-Ag2O@GO composite was modified through self-polymerization. (Methods) Acacetin molecularly imprinted polymers (AMIPs) were created via surface-initiated precipitation polymerization with acacetin as the template. These AMIPs were synthesized and characterized via ultraviolet (UV) spectroscopy, Fourier transform infrared (FT-IR) spectroscopy, scanning electron microscopy (SEM), X-ray diffraction (XRD), and transmission electron microscopy (TEM). Recycling experiments demonstrated the stability of the AMIPs. Analysis of the adsorption mechanism via the Langmuir and Freundlich models indicated that the Langmuir model was more suitable for predicting AMIPs. (Results) Scatchard plots showing two different binding sites of AMIPs for acacetin adsorption at various concentrations. The class selectivity of AMIPs for flavonoids was found in Acanthaceae ebracteatus. Compared with other analogs, competitive binding experiments revealed that the polymer had greater selectivity for acacetin. After AMIP solid-phase extraction (AMISPE), the linearity, LOD, LOQ, intraday and interday variabilities, recovery, and repeatability of AMIPs combined with HPLC–MS/MS were validated, confirming the method’s selectivity, precision, accuracy, and reproducibility. The contents of acacetin, apigenin, kaempferol, luteolin, quercetin, and genistein in Acanthaceae ebracteatus were determined. (Conclusions) These results indicate that AMISPE coupled with HPLC–MS/MS is a new, simple, and practical method for extracting, purifying, and enriching flavonoids from complex samples, such as those of herbs and other natural marine plants.