Interactions between the gut microbiome and mucosal immunoglobulins A, M and G in the developing infant gut

• Published in: bioRxiv
• Main Authors: Janzon, Anders, Goodrich, Julia K, Koren, Omry, The Teddy Study Group, Waters, Jillian, Ley, Ruth
• Format: Paper
• Language: English
• Published: Cold Spring Harbor Cold Spring Harbor Laboratory Press 15.05.2019; Cold Spring Harbor Laboratory
• Edition: 1.1
• Subjects: Age; Breast feeding; Breastfeeding & lactation; Coatings; Enterobacteriaceae; Flow cytometry; Immunoglobulin A; Immunoglobulin G; Immunoglobulin M; Infants; Intestinal microflora; Microbiology; Microbiota; Mucosa; rRNA 16S; IgA; gut microbiome; infant gut development; antibody coating; IgG; FACS; IgM
• ISSN: 2692-8205; 2692-8205
• DOI: 10.1101/637959

Objective: Interactions between the gut microbiome and immunoglobulin (Ig) A in infancy are important for future health. IgM and IgG are also present, however, their interactions with the microbiome in the developing infant are less understood. Design: We employed stool samples sampled 15 times in infancy from 32 healthy subjects at 4 locations in 3 countries (from the TEDDY study). We characterized patterns of microbiome development in relation to levels of IgA, IgG and IgM. For 8 infants from a single location, we FACS-sorted microbial cells from stool by Ig status. We used 16S rRNA gene profiling on full and sorted microbiomes to assess patterns of antibody coating in relation to age and other factors. Results: All antibodies decreased in concentration with age, but were augmented by breastmilk feeding regardless of infant age. Levels of IgA correlated with the relative abundances of OTUs belonging to the Bifidobacteria and Enterobacteriaceae, which dominated the early microbiome, and IgG levels correlated with Haemophilus. The diversity of Ig-coated microbiota was influenced by breastfeeding and age, but birth mode. IgA and IgM coated the same microbiota, while IgG targeted a different subset. Blautia generally evaded antibody coating, while members of the Bifidobacteria and Enterobacteriaceae were high in IgA/M. Conclusion: IgA/M have similar dynamics with respect to microbiome development with age, and their interactions with the microbiome are influenced by breastfeeding status. IgG generally does not coat the commensal microbiota.