Nanoscale subcellular architecture revealed by multicolor 3D salvaged fluorescence imaging

Combining the molecular specificity of fluorescent probes with three-dimensional (3D) imaging at nanoscale resolution is critical for investigating the spatial organization and interactions of cellular organelles and protein complexes. We present a super-resolution light microscope that enables simu...

Full description

Saved in:
Bibliographic Details
Published inbioRxiv
Main Authors Zhang, Yongdeng, Schroeder, Lena K, Lessard, Mark D, Kidd, Phylicia, Chung, Jeeyun, Song, Yuanbin, Benedetti, Lorena, Li, Yiming, Ries, Jonas, De Camilli, Pietro, Rothman, James E, Baddeley, David, Bewersdorf, Joerg
Format Paper
LanguageEnglish
Published Cold Spring Harbor Cold Spring Harbor Laboratory Press 18.04.2019
Cold Spring Harbor Laboratory
Edition1.1
Subjects
Biophysics
Electron microscopy
Endoplasmic reticulum
Fluorescent indicators
Golgi apparatus
Mammalian cells
Organelles
Online AccessGet full text

Cover

More Information
Summary:Combining the molecular specificity of fluorescent probes with three-dimensional (3D) imaging at nanoscale resolution is critical for investigating the spatial organization and interactions of cellular organelles and protein complexes. We present a super-resolution light microscope that enables simultaneous multicolor imaging of whole mammalian cells at ~20 nm 3D resolution. We show its power for cell biology research with fluorescence images that resolved the highly convoluted Golgi apparatus and the close contacts between the endoplasmic reticulum and the plasma membrane, structures that have traditionally been the imaging realm of electron microscopy.
Bibliography:SourceType-Working Papers-1
ObjectType-Working Paper/Pre-Print-1
content type line 50
ISSN:2692-8205
2692-8205
DOI:10.1101/613174