Propofol-Induced Unresponsiveness is Associated with Impaired Feedforward Connectivity in the Cortical Hierarchy

• Published in: bioRxiv
• Main Authors: Sanders, Robert D, Banks, Matthew I, Darracq, Matthieu, Moran, Rosalyn, Sleigh, Jamie, Gosseries, Olivia, Bonhomme, Vincent, Brichant, Jean-Francois, Rosanova, Mario, Raz, Aeyal, Tononi, Giulio, Massimini, Marcello, Laureys, Steven, Boly, Melanie
• Format: Paper
• Language: English
• Published: Cold Spring Harbor Cold Spring Harbor Laboratory Press 03.11.2017; Cold Spring Harbor Laboratory
• Edition: 1.1
• Subjects: Bayesian analysis; Consciousness; Cortex (parietal); EEG; Feedback; Information processing; Magnetic fields; Neuroscience; Occipital lobe; Propofol; Sleep and wakefulness; Theta rhythms; Transcranial magnetic stimulation; Visual cortex
• ISSN: 2692-8205; 2692-8205
• DOI: 10.1101/213504

Background: Impaired consciousness has been associated with impaired cortical signal propagation following transcranial magnetic stimulation (TMS). Herein we hypothesized that the reduced current propagation under propofol-induced unresponsiveness is associated with changes in both feedforward and feedback connectivity across the cortical hierarchy. Methods: Eight subjects underwent left occipital TMS coupled with high-density electroencephalograph (EEG) recordings during wakefulness and propofol-induced unconsciousness. Spectral analysis was applied to responses recorded from sensors overlying six hierarchical cortical sources involved in visual processing. Dynamic causal modelling (DCM) of evoked and induced source-space responses was used to investigate propofol effects on connectivity between regions. Results: Propofol produced a wideband reduction in evoked power following TMS in five out of six electrodes. Bayesian Model Selection supported a DCM with hierarchical feedforward and feedback connections to best fit the data. DCM of induced responses revealed that the primary effect of propofol was impaired feedforward responses in cross frequency theta/alpha-gamma coupling and within frequency theta coupling (F contrast, Family Wise Error corrected p<0.05). An exploratory analysis (thresholded at uncorrected p<0.001) also suggested that propofol impaired feedforward and feedback beta band coupling. Posthoc analyses showed impairments in all feedforward connections and one feedback connection from parietal to occipital cortex. DCM of the evoked response potential showed impaired feedforward connectivity between left sided occipital and parietal cortex (T contrast p=0.004, Bonferroni corrected). Conclusions: Our data suggest that propofol-induced loss of consciousness is associated with reduced evoked power and impaired hierarchical feedforward connectivity following occipital TMS.