Extremely bright, near-IR emitting spontaneously blinking fluorophores enable ratiometric multicolor nanoscopy in live cells

New bright, photostable, emission-orthogonal fluorophores that blink without toxic additives are needed to enable multi-color, live-cell, single-molecule localization microscopy (SMLM), especially for experiments that demand ultra-high-resolution live imaging. Here we report the design, synthesis, a...

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Main Authors Tyson, Jonathan, Hu, Kevin, Zheng, Shuai, Kidd, Phylicia, Dadina, Neville, Chu, Ling, Toomre, Derek, Bewersdorf, Joerg, Schepartz, Alanna
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LanguageEnglish
Published Cold Spring Harbor Laboratory 02.06.2021
Edition1.1
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Cell Biology
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ISSN2692-8205
DOI10.1101/2021.06.02.446776

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Abstract New bright, photostable, emission-orthogonal fluorophores that blink without toxic additives are needed to enable multi-color, live-cell, single-molecule localization microscopy (SMLM), especially for experiments that demand ultra-high-resolution live imaging. Here we report the design, synthesis, and biological evaluation of Yale676sb, a photostable, near-IR emitting fluorophore that achieves these goals in the context of an exceptional quantum yield (0.59). When used alongside HMSiR, Yale676sb enables simultaneous, live-cell, two-color SMLM of two intracellular organelles (ER + mitochondria) with only a single laser and no chemical additives.
AbstractList New bright, photostable, emission-orthogonal fluorophores that blink without toxic additives are needed to enable multi-color, live-cell, single-molecule localization microscopy (SMLM), especially for experiments that demand ultra-high-resolution live imaging. Here we report the design, synthesis, and biological evaluation of Yale676sb, a photostable, near-IR emitting fluorophore that achieves these goals in the context of an exceptional quantum yield (0.59). When used alongside HMSiR, Yale676sb enables simultaneous, live-cell, two-color SMLM of two intracellular organelles (ER + mitochondria) with only a single laser and no chemical additives.
Author Bewersdorf, Joerg
Chu, Ling
Zheng, Shuai
Hu, Kevin
Toomre, Derek
Schepartz, Alanna
Kidd, Phylicia
Dadina, Neville
Tyson, Jonathan
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Snippet New bright, photostable, emission-orthogonal fluorophores that blink without toxic additives are needed to enable multi-color, live-cell, single-molecule...
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SubjectTerms Cell Biology
Title Extremely bright, near-IR emitting spontaneously blinking fluorophores enable ratiometric multicolor nanoscopy in live cells
URI https://www.biorxiv.org/content/10.1101/2021.06.02.446776
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