Extremely bright, near-IR emitting spontaneously blinking fluorophores enable ratiometric multicolor nanoscopy in live cells

New bright, photostable, emission-orthogonal fluorophores that blink without toxic additives are needed to enable multi-color, live-cell, single-molecule localization microscopy (SMLM), especially for experiments that demand ultra-high-resolution live imaging. Here we report the design, synthesis, a...

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Published inbioRxiv
Main Authors Tyson, Jonathan, Hu, Kevin, Zheng, Shuai, Kidd, Phylicia, Dadina, Neville, Chu, Ling, Toomre, Derek, Bewersdorf, Joerg, Schepartz, Alanna
Format Paper
LanguageEnglish
Published Cold Spring Harbor Laboratory 02.06.2021
Edition1.1
Subjects
Cell Biology
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Summary:New bright, photostable, emission-orthogonal fluorophores that blink without toxic additives are needed to enable multi-color, live-cell, single-molecule localization microscopy (SMLM), especially for experiments that demand ultra-high-resolution live imaging. Here we report the design, synthesis, and biological evaluation of Yale676sb, a photostable, near-IR emitting fluorophore that achieves these goals in the context of an exceptional quantum yield (0.59). When used alongside HMSiR, Yale676sb enables simultaneous, live-cell, two-color SMLM of two intracellular organelles (ER + mitochondria) with only a single laser and no chemical additives.
Bibliography:Competing Interest Statement: The authors have declared no competing interest.
ISSN:2692-8205
DOI:10.1101/2021.06.02.446776