A scalable, fully automated process for construction of sequence-ready human exome targeted capture libraries

Genome targeting methods enable cost-effective capture of specific subsets of the genome for sequencing. We present here an automated, highly scalable method for carrying out the Solution Hybrid Selection capture approach that provides a dramatic increase in scale and throughput of sequence-ready li...

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Published inGenome biology Vol. 12; no. 1; p. R1
Main Authors Fisher, Sheila, Barry, Andrew, Abreu, Justin, Minie, Brian, Nolan, Jillian, Delorey, Toni M, Young, Geneva, Fennell, Timothy J, Allen, Alexander, Ambrogio, Lauren, Berlin, Aaron M, Blumenstiel, Brendan, Cibulskis, Kristian, Friedrich, Dennis, Johnson, Ryan, Juhn, Frank, Reilly, Brian, Shammas, Ramy, Stalker, John, Sykes, Sean M, Thompson, Jon, Walsh, John, Zimmer, Andrew, Zwirko, Zac, Gabriel, Stacey, Nicol, Robert, Nusbaum, Chad
Format Journal Article
LanguageEnglish
Published England BioMed Central Ltd 04.01.2011
BioMed Central
Subjects
Automation, Laboratory
Exome
Gene Library
Genome, Human
High-Throughput Nucleotide Sequencing
Humans
Method
Nucleic Acid Hybridization - methods
Oligonucleotide Array Sequence Analysis - methods
Quality Control
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Summary:Genome targeting methods enable cost-effective capture of specific subsets of the genome for sequencing. We present here an automated, highly scalable method for carrying out the Solution Hybrid Selection capture approach that provides a dramatic increase in scale and throughput of sequence-ready libraries produced. Significant process improvements and a series of in-process quality control checkpoints are also added. These process improvements can also be used in a manual version of the protocol.
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ISSN:1465-6906
1474-760X
1465-6914
DOI:10.1186/gb-2011-12-1-r1